論文 - 詳細
| RRC ID | 39900 |
|---|---|
| 著者 | Shoji Y, Inoue Y, Sugisawa H, Itou T, Endo T, Sakai T. |
| タイトル | Molecular cloning and functional characterization of bottlenose dolphin (Tursiops truncatus) tumor necrosis factor alpha. |
| ジャーナル | Vet Immunol Immunopathol |
| Abstract |
Bottlenose dolphin tumor necrosis factor alpha (doTNF-alpha) cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR) and the nucleic and deduced amino acid sequences were determined. The sequence of the cDNA clones shows that doTNF-alpha has an open reading frame of 699bp encoding 233 amino acids. The nucleic acid sequence of doTNF-alpha indicates 90, 88, 87, and 79% similarity with the cattle, pig, human, and mouse TNF-alpha gene, respectively. Based on the analysis of human and mouse TNF-alpha molecules, doTNF-alpha is processed to a mature protein with 157 amino acids. The 233 amino acids precursor has a hydrophobic region that could serve as a transmembrane domain. The recombinant doTNF-alpha expressed in Escherichia coli as a glutathione S-transferase fusion protein reacted with anti-human TNF-alpha antibody and exerted cytotoxity to the TNF-alpha sensitive murine cell line L929. |
| 巻・号 | 82(3-4) |
| ページ | 183-92 |
| 公開日 | 2001-10-1 |
| DOI | 10.1016/s0165-2427(01)00353-1 |
| PII | S0165242701003531 |
| PMID | 11587733 |
| MeSH | Amino Acid Sequence Animals Base Sequence Biological Assay / veterinary Cells, Cultured DNA / chemistry Dolphins / genetics* Dolphins / immunology* Escherichia coli / genetics Mice Molecular Sequence Data RNA / chemistry RNA / isolation & purification Random Amplified Polymorphic DNA Technique / veterinary Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Recombinant Proteins / toxicity Reverse Transcriptase Polymerase Chain Reaction / veterinary Sequence Homology, Amino Acid Tumor Necrosis Factor-alpha / genetics* Tumor Necrosis Factor-alpha / physiology Tumor Necrosis Factor-alpha / toxicity |
| IF | 1.713 |
| 引用数 | 18 |
| WOS 分野 | VETERINARY SCIENCES IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |