Reference - Detail
| RRC ID | 39901 |
|---|---|
| Author | Tamura HO, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F. |
| Title | Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues. |
| Journal | Biol Pharm Bull |
| Abstract |
To explore the physiological roles of sulfotransferases (SULTs) in extra-hepatic tissues, we examined the expression of eight SULT genes by reverse transcription (RT)-PCR in human cell lines that were established from various tissues. Expression levels of SULTs were low in neural cell lines such as NB-1 and GI-1, and high in epithelial cell lines, such as Caco-2 and BeWo. SULT1C2 expression was abundant in all cell types, whereas that of SULT1E1, SULTIBI or SULT2B1 was restricted to a specific cell type. SULT1C1, which can catalyze the sulfation of N-hydroxy-2-acetylaminofluorene, was expressed in Caco-2, BeWo and KB562. Induction of differentiation did not generally affect SULT expression, although that of SULT1C2 was reduced after differentiation of the neuroblastoma cell line, NB-1, was induced. The profile of SULT expression in the culture cells obtained here gives clues to understanding the physiological roles of SULT enzymes in extra-hepatic tissues or organs. |
| Volume | 24(11) |
| Pages | 1258-62 |
| Published | 2001-11-1 |
| DOI | 10.1248/bpb.24.1258 |
| PMID | 11725959 |
| MeSH | Caco-2 Cells / cytology Caco-2 Cells / metabolism* Cell Differentiation / physiology Choriocarcinoma / enzymology Colonic Neoplasms / enzymology Dopamine / metabolism Female Gene Expression Profiling HL-60 Cells / cytology HL-60 Cells / metabolism* Humans Intestinal Mucosa / enzymology Isoenzymes / biosynthesis Isoenzymes / genetics K562 Cells / cytology K562 Cells / metabolism* Naphthols / metabolism Neurons / cytology Neurons / enzymology Substrate Specificity Sulfotransferases / biosynthesis* Sulfotransferases / blood Sulfotransferases / genetics Uterine Neoplasms / enzymology |
| IF | 1.863 |
| Times Cited | 25 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Resource | |
| Human and Animal Cells | CACO-2(RCB0988) GI-1(RCB0763) HL60(RCB0041) |