| RRC ID |
39911
|
| 著者 |
Ikeda M, Hirokawa M, Satani N, Kinoshita T, Watanabe Y, Inoue H, Tone S, Ishikawa T, Minatogawa Y.
|
| タイトル |
Molecular cloning and characterization of a steroid receptor-binding regulator of G-protein signaling protein cDNA.
|
| ジャーナル |
Gene
|
| Abstract |
Steroid hormone receptors are composed of six major functional domains, i.e. the A/B domains as the activation function 1 domain (AF-1), domain C as the DNA-binding domain, domain D as a hinge domain and domain E/F as the ligand-dependent transcriptional domain (AF-2). They regulate gene transcription through interactions with various nuclear factors of their domains, such as AF-1 and AF-2. We have insufficient knowledge of the function of the DNA-binding domain (domain C) except for its DNA-binding function or the hinge domain (domain D). Therefore, we attempted to identify factors interacting with the domains by using a yeast two-hybrid system. Domains C and D of estrogen receptor alpha were used as a bait to isolate cDNA clones from a rat ovary cDNA library. We isolated the cDNA clone of a novel steroid receptor-binding protein bearing the regulator of G-protein signaling (RGS) designated as SRB-RGS. The protein repressed the transcriptional activity of estrogen receptor alpha, suggesting cross-talk of steroid hormones and peptide hormones (or growth factors) for signal transductions mediated by SRB-RGS.
|
| 巻・号 |
273(2)
|
| ページ |
207-14
|
| 公開日 |
2001-8-8
|
| DOI |
10.1016/s0378-1119(01)00589-3
|
| PII |
S0378111901005893
|
| PMID |
11595167
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Binding Sites / genetics
Blotting, Northern
COS Cells
Cloning, Molecular
DNA, Complementary / chemistry
DNA, Complementary / genetics*
DNA, Complementary / isolation & purification
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Female
Gene Expression Regulation
Gene Library
Molecular Sequence Data
Protein Binding
RGS Proteins
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Receptors, Estrogen / genetics
Receptors, Estrogen / metabolism
Receptors, Steroid / genetics
Receptors, Steroid / metabolism*
Repressor Proteins / genetics*
Repressor Proteins / metabolism
Saccharomyces cerevisiae / genetics
Sequence Analysis, DNA
Tissue Distribution
Transcription, Genetic
Two-Hybrid System Techniques
|
| IF |
2.984
|
| 引用数 |
11
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| ヒト・動物細胞 |
|
