論文 - 詳細
| RRC ID | 39911 |
|---|---|
| 著者 | Ikeda M, Hirokawa M, Satani N, Kinoshita T, Watanabe Y, Inoue H, Tone S, Ishikawa T, Minatogawa Y. |
| タイトル | Molecular cloning and characterization of a steroid receptor-binding regulator of G-protein signaling protein cDNA. |
| ジャーナル | Gene |
| Abstract |
Steroid hormone receptors are composed of six major functional domains, i.e. the A/B domains as the activation function 1 domain (AF-1), domain C as the DNA-binding domain, domain D as a hinge domain and domain E/F as the ligand-dependent transcriptional domain (AF-2). They regulate gene transcription through interactions with various nuclear factors of their domains, such as AF-1 and AF-2. We have insufficient knowledge of the function of the DNA-binding domain (domain C) except for its DNA-binding function or the hinge domain (domain D). Therefore, we attempted to identify factors interacting with the domains by using a yeast two-hybrid system. Domains C and D of estrogen receptor alpha were used as a bait to isolate cDNA clones from a rat ovary cDNA library. We isolated the cDNA clone of a novel steroid receptor-binding protein bearing the regulator of G-protein signaling (RGS) designated as SRB-RGS. The protein repressed the transcriptional activity of estrogen receptor alpha, suggesting cross-talk of steroid hormones and peptide hormones (or growth factors) for signal transductions mediated by SRB-RGS. |
| 巻・号 | 273(2) |
| ページ | 207-14 |
| 公開日 | 2001-8-8 |
| DOI | 10.1016/s0378-1119(01)00589-3 |
| PII | S0378111901005893 |
| PMID | 11595167 |
| MeSH | Amino Acid Sequence Animals Base Sequence Binding Sites / genetics Blotting, Northern COS Cells Cloning, Molecular DNA, Complementary / chemistry DNA, Complementary / genetics* DNA, Complementary / isolation & purification DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Female Gene Expression Regulation Gene Library Molecular Sequence Data Protein Binding RGS Proteins RNA, Messenger / genetics RNA, Messenger / metabolism Rats Receptors, Estrogen / genetics Receptors, Estrogen / metabolism Receptors, Steroid / genetics Receptors, Steroid / metabolism* Repressor Proteins / genetics* Repressor Proteins / metabolism Saccharomyces cerevisiae / genetics Sequence Analysis, DNA Tissue Distribution Transcription, Genetic Two-Hybrid System Techniques |
| IF | 2.984 |
| 引用数 | 11 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |