| RRC ID |
39917
|
| Author |
Kohda A, Taguchi H, Okumura K.
|
| Title |
Preparation of extended metaphase chromosomes from human cultured cells using a topoisomerase II inhibitor, ICRF-193.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Effects of ICRF-193, a topoisomerase II inhibitor, on metaphase chromosome preparations were examined. A short-time exposure of this drug to human HL60 cells in a suspension culture before harvest resulted in obtaining more extended metaphase chromosomes. The length of chromosome 6 identified by fluorescence in situ hybridization was twice as long with this drug treatment. Together with effectiveness for adherent HepG2 cells, these results suggest that treatments with ICRF-193 provide a simple and reliable method for extended metaphase chromosome preparations from cultured cells.
|
| Volume |
65(5)
|
| Pages |
1248-51
|
| Published |
2001-5-1
|
| DOI |
10.1271/bbb.65.1248
|
| PMID |
11440151
|
| MeSH |
Chromosomes, Human*
Diketopiperazines
Enzyme Inhibitors / pharmacology*
Humans
In Situ Hybridization, Fluorescence
Metaphase*
Piperazines / pharmacology*
Topoisomerase II Inhibitors*
Tumor Cells, Cultured
|
| IF |
1.516
|
| Times Cited |
2
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HL60(RCB0041) |
