論文 - 詳細
| RRC ID | 39921 |
|---|---|
| 著者 | Kondo A, Mogi M, Koshihara Y, Togari A. |
| タイトル | Signal transduction system for interleukin-6 and interleukin-11 synthesis stimulated by epinephrine in human osteoblasts and human osteogenic sarcoma cells. |
| ジャーナル | Biochem Pharmacol |
| Abstract |
Epinephrine increased gene- and protein-expression of interleukin-6 (IL-6) and interleukin-11 (IL-11), which are capable of stimulating the development of osteoclasts from their hematopoietic precursors, in human osteoblast (SaM-1) and human osteosarcoma (SaOS-2, HOS, and MG-63) cell lines. An increase in IL-6 and IL-11 synthesis in response to epinephrine appeared to be a common feature in osteoblastic cells, but the magnitude of expression was different in these cell lines. In HOS cells treated with epinephrine, increases of IL-6 and IL-11 synthesis were inhibited by timolol (a beta-blocker), H-89 (N-[2-((p-bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide; an inhibitor of protein kinase A (PKA)) and SB203580 [4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole; an inhibitor of p38 mitogen-activated protein kinase (MAPK)], but not by phentolamine (an alpha-blocker), calphostin C [an inhibitor of protein kinase C (PKC)], or PD98059 (2'-amino-3'-methoxyflavone; an inhibitor of classic MAPK), suggesting a common pathway mediated by beta-adrenergic receptors in the PKA and p38 systems involved in the signal transduction of IL-6 and IL-11. Furthermore, expression of both genes was inhibited by curcumin [an inhibitor of activating protein-1 (AP-1) activation], but not by pyrrolidine dithiocarbamate (PDTC) [an inhibitor of nuclear factor (NF)-kappaB]. The pharmacological study suggested that coinduction of the two genes in response to epinephrine occurred via activation of AP-1. The findings of the present study suggest that coinduction of IL-6 and IL-11 in response to epinephrine probably occurs via the PKA and p38 MAPK systems, leading to the transcriptional activation of AP-1 in human osteoblastic cells. |
| 巻・号 | 61(3) |
| ページ | 319-26 |
| 公開日 | 2001-2-1 |
| DOI | 10.1016/s0006-2952(00)00544-x |
| PII | S0006-2952(00)00544-X |
| PMID | 11172736 |
| MeSH | Adrenergic Agonists / pharmacology* Adrenergic alpha-Antagonists / pharmacology Adrenergic beta-Antagonists / pharmacology Antioxidants / pharmacology Curcumin / pharmacology Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors Drug Interactions Enzyme Inhibitors / pharmacology Enzyme-Linked Immunosorbent Assay Epinephrine / pharmacology* Gene Expression / drug effects Humans Interleukin-11 / biosynthesis* Interleukin-6 / biosynthesis* Mitogen-Activated Protein Kinases / antagonists & inhibitors NF-kappa B / antagonists & inhibitors Osteoblasts / drug effects* Osteoblasts / metabolism Osteosarcoma / metabolism* Osteosarcoma / pathology Protein Kinase C / antagonists & inhibitors Pyrrolidines / pharmacology RNA, Messenger / biosynthesis RNA, Messenger / drug effects Reverse Transcriptase Polymerase Chain Reaction Signal Transduction* / drug effects Thiocarbamates / pharmacology Transcription Factor AP-1 / antagonists & inhibitors Tumor Cells, Cultured |
| IF | 4.96 |
| 引用数 | 55 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Saos-2(RCB0428) |