RRC ID 39932
著者 Hatano H, Kawashima H, Ogose A, Hotta T, Endo N.
タイトル A PCR-ELISA assay for the detection of disseminated osteosarcoma cells in a mouse metastatic model.
ジャーナル J Orthop Sci
Abstract Circulating tumor cells in the blood play a central role in the metastatic process. There have been no reports describing the relationship between lung metastasis and circulating osteosarcoma cells. We developed a system with a polymerase chain reaction assay based on an enzyme-linked immunosorbent assay (PCR-ELISA) to detect circulating osteosarcoma cells in a mouse metastatic model. Osf2/Cbfa1, hereafter called Osf2, a member of the runt family of transcription factors, was used as a target gene. One splicing variant of Osf2 mRNA was identified and its expression was restricted to the bones and osteosarcomas. The amount of the splicing variant of Osf2 mRNA was significantly higher in the blood of mice with metastasis than in the blood of the control group. The PCR-ELISA using Osf2 mRNA is a potential method to detect circulating osteosarcoma cells in peripheral blood.
巻・号 6(3)
ページ 269-75
公開日 2001-1-1
DOI 10.1007/s007760100046
PII S0949-2658(15)33348-0
PMID 11484122
MeSH Alternative Splicing Animals Bone Neoplasms / blood Bone Neoplasms / genetics Bone Neoplasms / pathology* Core Binding Factor Alpha 1 Subunit Disease Models, Animal* Enzyme-Linked Immunosorbent Assay Gene Expression Profiling Lung Neoplasms / secondary Mice Mice, Inbred C3H Neoplasm Proteins* Neoplastic Cells, Circulating* Osteosarcoma / blood Osteosarcoma / genetics Osteosarcoma / secondary* Polymerase Chain Reaction / methods* RNA Splicing Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / isolation & purification Transcription Factors / metabolism* Tumor Cells, Cultured
IF 1.259
リソース情報
ヒト・動物細胞 OST(RCB0454) MOLT-4(RCB0206) A549(RCB0098) MDA-MB-453(RCB1192) Hep G2(RCB0459)