Reference - Detail
| RRC ID | 39934 |
|---|---|
| Author | Iwasaki T, Murata-Hori M, Ishitobi S, Hosoya H. |
| Title | Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells. |
| Journal | Cell Struct Funct |
| Abstract |
Activity of nonmuscle myosin II is regulated by phosphorylation of its regulatory light chain (MRLC). Phosphoryration of MRLC at both Thr18 and Ser19 (diphosphorylation) results in higher MgATPase activity and in promotion of the assembly of myosin II filaments than does that of MRLC at Ser19 (monophosphorylation) in vitro. To determine the roles of the diphosphorylated MRLC in vivo, we transfected three kinds of MRLC mutants, unphosphorylated, monophosphorylated and diphosphorylated forms (MRLC2(T18AS19A), substitution of both Ser19 and Thr18 by Ala; MRLC2(T18AS19D), Ser19 by Asp and Thr18 by Ala; and MRLC2(T18DS19D), both Ser19 and Thr18 by Asp, respectively), into HeLa cells. Cells overexpressing the mutant MRLC2(T18DS19D) contained a larger number of actin filament bundles than did those overexpressing the mutant MRLC2(T18AS19D). Moreover, cells overexpressing the nonphosphorylatable mutant MRLC2(T18AS19A) showed a decrease in the number of actin filament bundles. Taken together, our data suggest that diphosphorylation of MRLC plays an important role in regulating actin filament assembly and reorganization in nonmuscle cells. |
| Volume | 26(6) |
| Pages | 677-83 |
| Published | 2001-12-1 |
| DOI | 10.1247/csf.26.677 |
| PMID | 11942626 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cell Division* HeLa Cells Humans Immunohistochemistry Interphase* Molecular Sequence Data Myosin Light Chains / chemistry Myosin Light Chains / genetics Myosin Light Chains / metabolism* Myosin Type II / metabolism Phosphorylation Protein Isoforms Recombinant Proteins / genetics Recombinant Proteins / metabolism Stress Fibers / metabolism* |
| IF | 2.429 |
| Times Cited | 62 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) REF-RGB(RCB0165) |