| RRC ID |
39954
|
| Author |
Dohmoto K, Hojo S, Fujita J, Yang Y, Ueda Y, Bandoh S, Yamaji Y, Ohtsuki Y, Dobashi N, Ishida T, Takahara J.
|
| Title |
The role of caspase 3 in producing cytokeratin 19 fragment (CYFRA21-1) in human lung cancer cell lines.
|
| Journal |
Int J Cancer
|
| Abstract |
The CYFRA 21-1 assay, which detects cytokeratin 19 (CK19) fragment, is widely used as a tumor marker for lung cancer, particularly non-small cell lung cancer. However, the reason that some lung cancer cell lines release CYFRA 21-1 in culture supernatants and others do not remains unclear. We hypothesized that the release of CYFRA 21-1 might be related to the expression of CK19 and caspase 3. In order to prove this, the quantities of mRNA for CK19 were evaluated by the competitive reverse transcriptase-polymerase chain reaction (RT-PCR). CK19 protein synthesis was also evaluated by Western blotting and immunohistochemistry, and the levels of CYFRA 21-1 in the culture supernatant were measured by an immunoradiometric assay. The expression of mRNA for caspase 3 was evaluated by the RT-PCR, and caspase 3 protein synthesis was also evaluated by immunohistochemistry. In 13 lung cancer cell lines, the amounts of mRNA for CK19 correlated with the levels of CYFRA 21-1 in culture supernatants, results of Western blotting for CK19, and positivities of immunohistochemistry for CK19. In 5 cell lines that produced a significant amount of CYFRA 21-1, the level of CYFRA 21-1 correlated with the positivity of RT-PCR for caspase 3 and immunohistochmistry for caspase 3. This suggests that caspase 3 played a role in the formation of CYFRA 21-1. In addition, the specific inhibitor of caspase 3 significantly inhibited the release of CYFRA 21-1 in culture supernatants. In conclusion, we demonstrate that caspase 3, which cleaves several intermediate filaments and carries out cell apoptosis, played an important role in producing CYFRA 21-1 in human lung cancer cell lines.
|
| Volume |
91(4)
|
| Pages |
468-73
|
| Published |
2001-2-15
|
| DOI |
10.1002/1097-0215(200002)9999:9999<::aid-ijc1082>3.0.co;2-t
|
| PII |
10.1002/1097-0215(200002)9999:9999<::AID-IJC1082>3.0.CO;2-T
|
| PMID |
11251967
|
| MeSH |
Animals
Antibodies, Monoclonal / metabolism
Antigens, Neoplasm / biosynthesis*
Apoptosis
Biomarkers, Tumor
Blotting, Western
Caspase 3
Caspase 6
Caspases / biosynthesis
Caspases / physiology*
Electrophoresis, Polyacrylamide Gel
Humans
Immunohistochemistry
Keratin-19
Keratins / biosynthesis*
Lung Neoplasms / metabolism*
Mice
Models, Genetic
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Tumor Cells, Cultured
|
| IF |
5.145
|
| Times Cited |
51
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
LC2/AD
LU135
LU165 |
