| RRC ID |
39957
|
| 著者 |
Senoo M, Tsuchiya I, Matsumura Y, Mori T, Saito Y, Kato H, Okamoto T, Habu S.
|
| タイトル |
Transcriptional dysregulation of the p73L / p63 / p51 / p40 / KET gene in human squamous cell carcinomas: expression of Delta Np73L, a novel dominant-negative isoform, and loss of expression of the potential tumour suppressor p51.
|
| ジャーナル |
Br J Cancer
|
| Abstract |
We have recently identified a second p53 -related p73L gene, also referred to as p63 / p51 / p40 / KET gene, which encodes the 2 major isoforms p73L and p51 resulting from different exon usage at their amino terminal regions. Although p73L and p51 are suspected to play oncogenic and tumour suppressive roles in mammalian cells, respectively, no evidence of linkage between the expression of these isoforms and human cancers has been reported so far. In this study, we first investigated the expression profile of p51 and p73L in various human tumour cell lines and found that a novel isoform, termed DeltaNp73L, was predominantly expressed in squamous cell carcinomas. The expression profile of DeltaNp73L/p73L/p51 in primary human skin cancer specimens showed that the expression of p51 was frequently lost (62%) but was detected in all normal skin samples. In p51-expressing skin cancers, DeltaNp73L expression was associated at a high frequency (75%) though it was not detected in normal skin tissues. Transient co-transfection data indicate the possibility that DeltaNp73L can inhibit p53-, and more preferentially, p51-mediated transactivation. These data suggest that the loss of expression of p51 and/or the expression of DeltaNp73L might contribute to the pathogenesis of human squamous cell carcinomas.
|
| 巻・号 |
84(9)
|
| ページ |
1235-41
|
| 公開日 |
2001-5-4
|
| DOI |
10.1054/bjoc.2000.1735
|
| PII |
S000709200091735X
|
| PMID |
11336476
|
| PMC |
PMC2363892
|
| MeSH |
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / physiopathology
Cell Line
DNA-Binding Proteins / biosynthesis*
DNA-Binding Proteins / genetics
Gene Expression Regulation, Neoplastic*
Genes, Tumor Suppressor
Humans
Membrane Proteins*
NADPH Oxidases
Nuclear Proteins / biosynthesis*
Nuclear Proteins / genetics
Phosphoproteins / biosynthesis*
Phosphoproteins / genetics
Proteins*
Skin Neoplasms / genetics*
Skin Neoplasms / physiopathology
Trans-Activators*
Transcription Factors
Transcription, Genetic
Tumor Protein p73
Tumor Suppressor Protein p53 / biosynthesis*
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Proteins
|
| IF |
5.791
|
| 引用数 |
49
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MKN1(RCB1003)
MKN74(RCB1002)
COS-7(RCB0539) |
