| RRC ID |
39959
|
| Author |
Nadano D, Aoki C, Yoshinaka T, Irie S, Sato TA.
|
| Title |
Electrophoretic characterization of ribosomal subunits and proteins in apoptosis: specific downregulation of S11 in staurosporine-treated human breast carcinoma cells.
|
| Journal |
Biochemistry
|
| Abstract |
Stimulation of death receptors (Fas on human T-cell leukemia Jurkat cells and tumor necrosis factor receptor-1 on human monoblastic leukemia U937 cells) triggers the specific degradation of 28S ribosomal RNA, and this process may contribute to cell death through the inhibition of protein synthesis. We have developed an analytical method using a polyacrylamide-agarose composite gel to evaluate ribosomal subunits in apoptotic cells (human breast carcinoma MCF-7 cells treated with staurosporine and human 293T cells irradiated with ultraviolet light were used in addition to the two apoptosis systems described above). No alterations were detected by this method, suggesting that apoptosis, including the process of ribosomal RNA degradation, does not cause fragmentation or extensive conformational changes in the ribosome. We also examined the status of 21 different ribosomal proteins in apoptotic cells by immunoblotting with polyclonal antibodies. S11 was specifically downregulated in apoptotic MCF-7 cells and in other apoptotic breast carcinoma cells. Previous studies have shown that S11 is heterogeneously expressed in cancer cells. Taken together, it appears that particular intracellular environments regulate the expression of S11 protein. However, the mechanism by which this process is modulated is as yet unknown. Furthermore, we have demonstrated that our composite gel electrophoresis system can efficiently detect ubiquitination of ribosomal subunits.
|
| Volume |
40(50)
|
| Pages |
15184-93
|
| Published |
2001-12-18
|
| DOI |
10.1021/bi0108397
|
| PII |
bi0108397
|
| PMID |
11735401
|
| MeSH |
Amino Acid Sequence
Animals
Apoptosis
Breast Neoplasms / drug therapy*
Breast Neoplasms / genetics
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology
Down-Regulation / drug effects
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Female
Humans
Jurkat Cells
Male
Molecular Sequence Data
Rats
Rats, Sprague-Dawley
Ribosomal Proteins / genetics
Ribosomal Proteins / metabolism*
Ribosomes / drug effects
Ribosomes / metabolism
Staurosporine / pharmacology*
Tumor Cells, Cultured
U937 Cells
Ubiquitin / metabolism
|
| IF |
2.865
|
| Times Cited |
21
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
NEC8(RCB0489) |
