RRC ID |
39965
|
著者 |
Sakakura C, Hagiwara A, Yasuoka R, Fujita Y, Nakanishi M, Masuda K, Shimomura K, Nakamura Y, Inazawa J, Abe T, Yamagishi H.
|
タイトル |
Tumour-amplified kinase BTAK is amplified and overexpressed in gastric cancers with possible involvement in aneuploid formation.
|
ジャーナル |
Br J Cancer
|
Abstract |
Our recent analysis of gastric cancers using comparative genomic hybridization (CGH) revealed a novel high frequent copy number increase in the long arm of chromosome 20. Tumour-amplified kinase BTAK was recently cloned from breast cancers and mapped on 20q13 as a target gene for this amplification in human breast cancers. In the study presented here, we analysed BTAK copy-number and expression, and their relation to the ploidy pattern in 72 primary gastric cancers. Furthermore, wild-type BTAK and its deletion mutants were transfected to gastric cancers to examine changes in cell proliferation and DNA ploidy pattern. Evaluation of 72 unselected primary gastric cancers found BTAK amplification in 5% and overexpression in more than 50%. All four clinical samples with BTAK amplification showed aneuploidy and poor prognosis. Transfection of BTAK in near-diploid gastric cancers induced another aneuploid cell population. In contrast, the c-terminal-deleted mutant of BTAK induced no effect in DNA ploidy pattern and inhibited gastric cancer cell proliferation. These results suggest that BTAK may be involved in gastric cancer cell aneuploid formation, and is a candidate gene for the increase in the number of copies of the 20q, and thus may contribute to an increase in the malignant phenotype of gastric cancer.
|
巻・号 |
84(6)
|
ページ |
824-31
|
公開日 |
2001-3-23
|
DOI |
10.1054/bjoc.2000.1684
|
PII |
S0007092000916847
|
PMID |
11259099
|
PMC |
PMC2363814
|
MeSH |
Adult
Aged
Aged, 80 and over
Aneuploidy*
Aurora Kinase A
Aurora Kinases
Base Sequence
DNA Primers
Humans
Immunoblotting
In Situ Hybridization, Fluorescence
Male
Middle Aged
Protein Serine-Threonine Kinases / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms / enzymology
Stomach Neoplasms / genetics*
Stomach Neoplasms / pathology
|
IF |
5.791
|
引用数 |
193
|
WOS 分野
|
ONCOLOGY
|
リソース情報 |
ヒト・動物細胞 |
MKN1(RCB1003)
MKN28(RCB1000)
MKN45(RCB1001)
MKN74(RCB1002) |