RRC ID 39977
Author Kiyokawa N, Mori T, Taguchi T, Saito M, Mimori K, Suzuki T, Sekino T, Sato N, Nakajima H, Katagiri YU, Takeda T, Fujimoto J.
Title Activation of the caspase cascade during Stx1-induced apoptosis in Burkitt's lymphoma cells.
Journal J Cell Biochem
Abstract Shiga toxin 1 (Stx1) produced by Escherichia coli has been reported to induce apoptosis in many different cell types, including Burkitt's lymphoma (BL) cells. Since it has been established that the caspases play essential roles as the effector molecules in the apoptotic process in most cases, we examined the kinetics of caspase activation during the process of Stx1-mediated apoptosis of BL cells. Using Ramos BL cells that are highly sensitive to Stx1-mediated cytotoxicity, we observed that multiple caspases, including caspase-3, -7, and -8 were promptly activated following Stx1 treatment, as indicated by both the procaspase cleavages and enhancement of cleavage of the tetrapeptide substrates of the caspases. In addition, the inhibition assay revealed that caspase-8 is located upstream of both caspase-3 and -7, suggesting that Stx1-mediated apoptosis utilizes a similar caspase cascade to that involved in Fas-mediated apoptosis. Neither anti-Fas mAb nor TNF-alpha, however, affected the Stx1-mediated apoptosis of Ramos cells. Although the precise mechanism of Stx1-mediated activation of caspase-8 is still unclear, we have demonstrated that crosslinkage of CD77, a functional receptor for Stx1, with specific antibody is sufficient to induce activation of caspase-8. Our findings should provide new insight into the understanding of the molecular basis of Stx1-mediated cell injury.
Volume 81(1)
Pages 128-42
Published 2001-1-1
DOI 10.1002/1097-4644(20010401)81:1<128::aid-jcb1029>3.0.co;2-g
PII 10.1002/1097-4644(20010401)81:1<128::AID-JCB1029>3.0.CO;2-G
PMID 11180403
MeSH Antibodies, Monoclonal / immunology Antibodies, Monoclonal / pharmacology Apoptosis / drug effects* Blotting, Western Burkitt Lymphoma / enzymology* Burkitt Lymphoma / pathology Caspase Inhibitors Caspases / metabolism* Cysteine Proteinase Inhibitors / pharmacology Enzyme Activation Fluorescent Antibody Technique Humans Peptides / pharmacology Shiga Toxin 1 / pharmacology* Tumor Cells, Cultured fas Receptor / immunology
IF 3.448
Times Cited 35
Human and Animal Cells