RRC ID 39986
Author Fidelin K, Djenoune L, Stokes C, Prendergast A, Gomez J, Baradel A, Del Bene F, Wyart C.
Title State-Dependent Modulation of Locomotion by GABAergic Spinal Sensory Neurons.
Journal Curr. Biol.
Abstract The cerebrospinal fluid (CSF) constitutes an interface through which chemical cues can reach and modulate the activity of neurons located at the epithelial boundary within the entire nervous system. Here, we investigate the role and functional connectivity of a class of GABAergic sensory neurons contacting the CSF in the vertebrate spinal cord and referred to as CSF-cNs. The remote activation of CSF-cNs was shown to trigger delayed slow locomotion in the zebrafish larva, suggesting that these cells modulate components of locomotor central pattern generators (CPGs). Combining anatomy, electrophysiology, and optogenetics in vivo, we show that CSF-cNs form active GABAergic synapses onto V0-v glutamatergic interneurons, an essential component of locomotor CPGs. We confirmed that activating CSF-cNs at rest induced delayed slow locomotion in the fictive preparation. In contrast, the activation of CSF-cNs promptly inhibited ongoing slow locomotion. Moreover, selective activation of rostral CSF-cNs during ongoing activity disrupted rostrocaudal propagation of descending excitation along the spinal cord, indicating that CSF-cNs primarily act at the premotor level. Altogether, our results demonstrate how a spinal GABAergic sensory neuron can tune the excitability of locomotor CPGs in a state-dependent manner by projecting onto essential components of the excitatory premotor pool.
Volume 25(23)
Pages 3035-47
Published 2015-12-7
DOI 10.1016/j.cub.2015.09.070
PII S0960-9822(15)01224-5
PMID 26752076
MeSH Animals Cerebrospinal Fluid / physiology GABAergic Neurons / physiology* Interneurons / physiology Locomotion* Sensory Receptor Cells / physiology* Spinal Cord / physiology Synapses / physiology Zebrafish / physiology*
IF 9.193
Times Cited 40
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Zebrafish Tg(vglut2a:lRl-GFP) Tg(gad1b:GFP)