Author Narita M, Wang Y, Kita A, Omi N, Yamada Y, Hiai H.
Title Genetic analysis of Nakano Cataract and its modifier genes in mice.
Journal Exp Eye Res
Abstract The Nakano Cataract (NCT) is an autosomal, recessive, single gene mutation in mice leading to an osmotic cataract induced by an endogenous inhibitor of Na, K-ATPase. In this report, we further refined the map position of the mutant locus to a <0.7c M segment between D16Mit5 and D16Mit185 in 1,000 BALB/c-nct/nct x(BALB/c- nct/nctxMSM)F1 backcrossed mice with PCR-based microsatellite analysis. The NCT in the original Nakano mice developed at 3 weeks of age, rapidly formed a pin-head type dense opacity, whereas the cataract in the congenic BALB/c- nct/nct mice developed at 5-6 weeks of age or later, slowly formed a diffuse opacity. A major histological difference was the presence or absence of heavy condensation of the lens nucleus. These two types of cataract were segregated in the backcrossed mice. Linkage analysis of the two subtypes among the backcrossed mice revealed two recessive BALB/c-derived modifier genes on chromosome 3 and 10.
Volume 75(6)
Pages 745-51
Published 2002-12-1
DOI 10.1006/exer.2002.2068
PII S0014483502920683
PMID 12470976
MeSH Animals Cataract / genetics* Cataract / metabolism Cataract / pathology Chromosome Mapping Crosses, Genetic Crystallins / analysis Disease Models, Animal Electrophoresis, Polyacrylamide Gel Genes, Recessive* Genetic Linkage Haplotypes Mice Mice, Inbred BALB C Microsatellite Repeats Mutation*
IF 3.011
Times Cited 9
Mice RBRC00422