論文 - 詳細
| RRC ID | 40000 |
|---|---|
| 著者 | Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC. |
| タイトル | Recoverin depletion accelerates cone photoresponse recovery. |
| ジャーナル | Open Biol |
| Abstract |
The neuronal Ca(2+)-binding protein Recoverin has been shown to regulate phototransduction termination in mammalian rods. Here we identify four recoverin genes in the zebrafish genome, rcv1a, rcv1b, rcv2a and rcv2b, and investigate their role in modulating the cone phototransduction cascade. While Recoverin-1b is only found in the adult retina, the other Recoverins are expressed throughout development in all four cone types, except Recoverin-1a, which is expressed only in rods and UV cones. Applying a double flash electroretinogram (ERG) paradigm, downregulation of Recoverin-2a or 2b accelerates cone photoresponse recovery, albeit at different light intensities. Exclusive recording from UV cones via spectral ERG reveals that knockdown of Recoverin-1a alone has no effect, but Recoverin-1a/2a double-knockdowns showed an even shorter recovery time than Recoverin-2a-deficient larvae. We also showed that UV cone photoresponse kinetics depend on Recoverin-2a function via cone-specific kinase Grk7a. This is the first in vivo study demonstrating that cone opsin deactivation kinetics determine overall photoresponse shut off kinetics. |
| 巻・号 | 5(8) |
| 公開日 | 2015-8-1 |
| DOI | 10.1098/rsob.150086 |
| PII | rsob.150086 |
| PMID | 26246494 |
| PMC | PMC4554923 |
| MeSH | Animals G-Protein-Coupled Receptor Kinases / metabolism Gene Expression Gene Knockdown Techniques Molecular Sequence Data Organ Specificity / genetics Recoverin / deficiency Recoverin / genetics Recoverin / metabolism* Retinal Cone Photoreceptor Cells / physiology* Retinal Cone Photoreceptor Cells / radiation effects Ultraviolet Rays Zebrafish Zebrafish Proteins |
| IF | 4.931 |
| 引用数 | 11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(zfSWS1-5.5A:EGFP)/kj9 Tg(RH2-2:GFP-PAC)/kj4 / tkk004 Tg(zfSWS2-3.5A:EGFP)/kj11 / tkk008 |