RRC ID 40017
著者 Morris JK, Chomyk A, Song P, Parker N, Deckard S, Trapp BD, Pimplikar SW, Dutta R.
タイトル Decrease in levels of the evolutionarily conserved microRNA miR-124 affects oligodendrocyte numbers in Zebrafish, Danio rerio.
ジャーナル Invert Neurosci
Abstract Oligodendrocytes produce multi-lamellar myelin membranes that surround axons in the central nervous system (CNS). Preservation and generation of myelin are potential therapeutic targets for dysmyelinating and demyelinating diseases. MicroRNAs (miRNAs) play a vital role in oligodendrocyte differentiation and overall CNS development. miR-124 is a well-conserved neuronal miRNA with important roles in neuronal differentiation and function. miR-124 levels increase following loss of myelin in both human and rodent brains. While the role of neuronal miR-124 in neurogenesis has been established, its effects on axonal outgrowth and oligodendrocytes are not currently known. We therefore explored the possible effect of selective knockdown of miR-124 in Danio rerio using a morpholino-based knockdown approach. No morphological abnormalities or loss of motor neurons were detected despite loss of axonal outgrowth. Morpholino-based knockdown of miR-124 led to reciprocal increases in mRNA levels of target genes that inhibit axonal and dendritic projections. Importantly, loss of miR-124 led to decreased oligodendrocyte cell numbers and myelination of axonal projections in the ventral hindbrain. Taken together, our results add a new dimension to the existing complexity of neuron-glial relationships and highlight the utility of Danio rerio as a model system to investigate such interactions.
巻・号 15(3)
ページ 4
公開日 2015-9-1
DOI 10.1007/s10158-015-0180-1
PMID 26159098
MeSH Animals Animals, Genetically Modified Dose-Response Relationship, Drug Embryo, Nonmammalian Gene Expression Regulation, Developmental / drug effects Gene Expression Regulation, Developmental / physiology* Larva MicroRNAs / genetics MicroRNAs / metabolism* Morpholinos / pharmacology Oligodendroglia / drug effects Oligodendroglia / metabolism* Zebrafish / anatomy & histology*
IF 0.556
引用数 8
WOS 分野 NEUROSCIENCES
リソース情報
ゼブラフィッシュ Tg(isl1:GFP)/rw0