論文 - 詳細
| RRC ID | 40017 |
|---|---|
| 著者 | Morris JK, Chomyk A, Song P, Parker N, Deckard S, Trapp BD, Pimplikar SW, Dutta R. |
| タイトル | Decrease in levels of the evolutionarily conserved microRNA miR-124 affects oligodendrocyte numbers in Zebrafish, Danio rerio. |
| ジャーナル | Invert Neurosci |
| Abstract |
Oligodendrocytes produce multi-lamellar myelin membranes that surround axons in the central nervous system (CNS). Preservation and generation of myelin are potential therapeutic targets for dysmyelinating and demyelinating diseases. MicroRNAs (miRNAs) play a vital role in oligodendrocyte differentiation and overall CNS development. miR-124 is a well-conserved neuronal miRNA with important roles in neuronal differentiation and function. miR-124 levels increase following loss of myelin in both human and rodent brains. While the role of neuronal miR-124 in neurogenesis has been established, its effects on axonal outgrowth and oligodendrocytes are not currently known. We therefore explored the possible effect of selective knockdown of miR-124 in Danio rerio using a morpholino-based knockdown approach. No morphological abnormalities or loss of motor neurons were detected despite loss of axonal outgrowth. Morpholino-based knockdown of miR-124 led to reciprocal increases in mRNA levels of target genes that inhibit axonal and dendritic projections. Importantly, loss of miR-124 led to decreased oligodendrocyte cell numbers and myelination of axonal projections in the ventral hindbrain. Taken together, our results add a new dimension to the existing complexity of neuron-glial relationships and highlight the utility of Danio rerio as a model system to investigate such interactions. |
| 巻・号 | 15(3) |
| ページ | 4 |
| 公開日 | 2015-9-1 |
| DOI | 10.1007/s10158-015-0180-1 |
| PMID | 26159098 |
| MeSH | Animals Animals, Genetically Modified Dose-Response Relationship, Drug Embryo, Nonmammalian Gene Expression Regulation, Developmental / drug effects Gene Expression Regulation, Developmental / physiology* Larva MicroRNAs / genetics MicroRNAs / metabolism* Morpholinos / pharmacology Oligodendroglia / drug effects Oligodendroglia / metabolism* Zebrafish / anatomy & histology* |
| IF | 0.556 |
| 引用数 | 8 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(isl1:GFP)/rw0 |