RRC ID 40025
Author Friedmann D, Hoagland A, Berlin S, Isacoff EY.
Title A spinal opsin controls early neural activity and drives a behavioral light response.
Journal Curr. Biol.
Abstract Nonvisual detection of light by the vertebrate hypothalamus, pineal, and retina is known to govern seasonal and circadian behaviors. However, the expression of opsins in multiple other brain structures suggests a more expansive repertoire for light regulation of physiology, behavior, and development. Translucent zebrafish embryos express extraretinal opsins early on, at a time when spontaneous activity in the developing CNS plays a role in neuronal maturation and circuit formation. Though the presence of extraretinal opsins is well documented, the function of direct photoreception by the CNS remains largely unknown. Here, we show that early activity in the zebrafish spinal central pattern generator (CPG) and the earliest locomotory behavior are dramatically inhibited by physiological levels of environmental light. We find that the photosensitivity of this circuit is conferred by vertebrate ancient long opsin A (VALopA), which we show to be a Gα(i)-coupled receptor that is expressed in the neurons of the spinal network. Sustained photoactivation of VALopA not only suppresses spontaneous activity but also alters the maturation of time-locked correlated network patterns. These results uncover a novel role for nonvisual opsins and a mechanism for environmental regulation of spontaneous motor behavior and neural activity in a circuit previously thought to be governed only by intrinsic developmental programs.
Volume 25(1)
Pages 69-74
Published 2015-1-5
DOI 10.1016/j.cub.2014.10.055
PII S0960-9822(14)01364-5
PMID 25484291
PMC PMC4286461
MeSH Animals Behavior, Animal / radiation effects* Central Nervous System / metabolism Central Nervous System / radiation effects* Embryo, Nonmammalian / metabolism Embryo, Nonmammalian / radiation effects* Light Motor Activity / radiation effects* Opsins / metabolism* Receptors, G-Protein-Coupled / metabolism* Xenopus Zebrafish
IF 9.251
Times Cited 7
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Zebrafish Tg(huC:GVP)