| RRC ID |
40043
|
| 著者 |
Tanaka N, Ishii H, Yin C, Koyama M, Sakuma Y, Kato M.
|
| タイトル |
Voltage-gated Ca2+ channel mRNAs and T-type Ca2+ currents in rat gonadotropin-releasing hormone neurons.
|
| ジャーナル |
J Physiol Sci
|
| Abstract |
Gonadotropin-releasing hormone (GnRH) neurons play a pivotal role in the neuroendocrine regulation of reproduction. We have previously reported that rat GnRH neurons exhibit voltage-gated Ca(2+) currents. In this study, oligo-cell RT-PCR was carried out to identify subtypes of the alpha(1) subunit of voltage-gated Ca(2+) channels in adult rat GnRH neurons. GnRH neurons expressed mRNAs for all five types of voltage-gated Ca(2+) channels. For T-type Ca(2+) channels, alpha(1H) was dominantly expressed in GnRH neurons. Electrophysiological analysis in acute slice preparations revealed that GnRH neurons from adult rats exhibited T-type Ca(2+) currents with fast inactivation kinetics (~20 ms at -30 mV) and a time constant of recovery from inactivation of ~0.6 s. These results indicate that rat GnRH neurons express subtypes of the alpha(1) subunit for all five types of voltage-gated Ca(2+) channel, and that alpha(1H) was the dominant subtype in T-type Ca(2+) channels.
|
| 巻・号 |
60(3)
|
| ページ |
195-204
|
| 公開日 |
2010-5-1
|
| DOI |
10.1007/s12576-010-0085-z
|
| PMID |
20101487
|
| MeSH |
Animals
Calcium / metabolism*
Calcium / pharmacology
Gonadotropin-Releasing Hormone / metabolism*
Gonadotropin-Releasing Hormone / pharmacology
Neurons / cytology
Neurons / metabolism
Neurons / physiology*
RNA, Messenger / metabolism*
Rats
Rats, Transgenic
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
2.804
|
| 引用数 |
12
|
|
WOS 分野
|
PHYSIOLOGY
|
| リソース情報 |
| ラット |
W-Tg(Gnrh1-EGFP)Nphy (strainID=704) |
