論文 - 詳細
| RRC ID | 4007 |
|---|---|
| 著者 | Lee MH, Kim B, Song SK, Heo JO, Yu NI, Lee SA, Kim M, Kim DG, Sohn SO, Lim CE, Chang KS, Lee MM, Lim J. |
| タイトル | Large-scale analysis of the GRAS gene family in Arabidopsis thaliana. |
| ジャーナル | Plant Mol Biol |
| Abstract |
GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development. |
| 巻・号 | 67(6) |
| ページ | 659-70 |
| 公開日 | 2008-8-1 |
| DOI | 10.1007/s11103-008-9345-1 |
| PMID | 18500650 |
| MeSH | Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / classification Arabidopsis Proteins / genetics* Arabidopsis Proteins / physiology* Cell Nucleus / chemistry Gene Expression Multigene Family Mutation Plants, Genetically Modified / metabolism RNA, Messenger / metabolism Transcription Factors / classification Transcription Factors / genetics* Transcription Factors / physiology* Two-Hybrid System Techniques |
| IF | 3.302 |
| 引用数 | 101 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst16033 pst13527 pst12157 pst15116 pst14184 pst10499 pst10702 |