RRC ID 40084
著者 Sakai K, Komaki H, Gonoi T.
タイトル Identification and Functional Analysis of the Nocardithiocin Gene Cluster in Nocardia pseudobrasiliensis.
ジャーナル PLoS One
Abstract Nocardithiocin is a thiopeptide compound isolated from the opportunistic pathogen Nocardia pseudobrasiliensis. It shows a strong activity against acid-fast bacteria and is also active against rifampicin-resistant Mycobacterium tuberculosis. Here, we report the identification of the nocardithiocin gene cluster in N. pseudobrasiliensis IFM 0761 based on conserved thiopeptide biosynthesis gene sequence and the whole genome sequence. The predicted gene cluster was confirmed by gene disruption and complementation. As expected, strains containing the disrupted gene did not produce nocardithiocin while gene complementation restored nocardithiocin production in these strains. The predicted cluster was further analyzed using RNA-seq which showed that the nocardithiocin gene cluster contains 12 genes within a 15.2-kb region. This finding will promote the improvement of nocardithiocin productivity and its derivatives production.
巻・号 10(11)
ページ e0143264
公開日 2015-1-1
DOI 10.1371/journal.pone.0143264
PII PONE-D-15-27385
PMID 26588225
PMC PMC4654471
MeSH Anti-Bacterial Agents / biosynthesis DNA Primers Genetic Complementation Test Genome, Bacterial Mass Spectrometry Multigene Family* Mycobacterium / drug effects Nocardia / genetics* Nocardia / metabolism Peptides / chemistry Peptides, Cyclic / genetics* Peptides, Cyclic / metabolism* Sequence Analysis, DNA Sequence Analysis, RNA Species Specificity Spectrophotometry, Ultraviolet Thiazoles / metabolism*
IF 2.74
引用数 11
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 IFM 0623 IFM 0624 IFM 0756 IFM 0757 IFM 0758 IFM 0759 IFM 0760 IFM 0761 IFM 0896 IFM 10098 IFM 10099 IFM 10700 IFM 11475 IFM 11477