論文 - 詳細
| RRC ID | 40093 |
|---|---|
| 著者 | Winkelströter LK, Bom VL, de Castro PA, Ramalho LN, Goldman MH, Brown NA, Rajendran R, Ramage G, Bovier E, Dos Reis TF, Savoldi M, Hagiwara D, Goldman GH. |
| タイトル | High osmolarity glycerol response PtcB phosphatase is important for Aspergillus fumigatus virulence. |
| ジャーナル | Mol Microbiol |
| Abstract |
Aspergillus fumigatus is a fungal pathogen that is capable of adapting to different host niches and to avoid host defenses. An enhanced understanding of how, and which, A. fumigatus signal transduction pathways are engaged in the regulation of these processes is essential for the development of improved disease control strategies. Protein phosphatases are central to numerous signal transduction pathways. To comprehend the functions of protein phosphatases in A. fumigatus, 32 phosphatase catalytic subunit encoding genes were identified. We have recognized PtcB as one of the phosphatases involved in the high osmolarity glycerol response (HOG) pathway. The ΔptcB mutant has both increased phosphorylation of the p38 MAPK (SakA) and expression of osmo-dependent genes. The ΔptcB strain was more sensitive to cell wall damaging agents, had increased chitin and β-1,3-glucan, and impaired biofilm formation. The ΔptcB strain was avirulent in a murine model of invasive pulmonary aspergillosis. These results stress the importance of the HOG pathway in the regulation of pathogenicity determinants and virulence in A. fumigatus. |
| 巻・号 | 96(1) |
| ページ | 42-54 |
| 公開日 | 2015-4-1 |
| DOI | 10.1111/mmi.12919 |
| PMID | 25597841 |
| MeSH | Animals Aspergillus fumigatus / genetics Aspergillus fumigatus / pathogenicity* Aspergillus fumigatus / physiology* Aspergillus fumigatus / ultrastructure Biofilms / growth & development Cell Wall / metabolism Chitin / metabolism Computational Biology Disease Models, Animal Fungal Proteins / genetics Fungal Proteins / metabolism Gene Expression Regulation, Fungal* Glycerol / metabolism* Mice Mutation Osmolar Concentration* Phosphoric Monoester Hydrolases / genetics* Phosphoric Monoester Hydrolases / metabolism Signal Transduction beta-Glucans / metabolism |
| IF | 3.418 |
| 引用数 | 29 |
| WOS 分野 | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | |