RRC ID 40093
Author Winkelströter LK, Bom VL, de Castro PA, Ramalho LN, Goldman MH, Brown NA, Rajendran R, Ramage G, Bovier E, Dos Reis TF, Savoldi M, Hagiwara D, Goldman GH.
Title High osmolarity glycerol response PtcB phosphatase is important for Aspergillus fumigatus virulence.
Journal Mol Microbiol
Abstract Aspergillus fumigatus is a fungal pathogen that is capable of adapting to different host niches and to avoid host defenses. An enhanced understanding of how, and which, A. fumigatus signal transduction pathways are engaged in the regulation of these processes is essential for the development of improved disease control strategies. Protein phosphatases are central to numerous signal transduction pathways. To comprehend the functions of protein phosphatases in A. fumigatus, 32 phosphatase catalytic subunit encoding genes were identified. We have recognized PtcB as one of the phosphatases involved in the high osmolarity glycerol response (HOG) pathway. The ΔptcB mutant has both increased phosphorylation of the p38 MAPK (SakA) and expression of osmo-dependent genes. The ΔptcB strain was more sensitive to cell wall damaging agents, had increased chitin and β-1,3-glucan, and impaired biofilm formation. The ΔptcB strain was avirulent in a murine model of invasive pulmonary aspergillosis. These results stress the importance of the HOG pathway in the regulation of pathogenicity determinants and virulence in A. fumigatus.
Volume 96(1)
Pages 42-54
Published 2015-4-1
DOI 10.1111/mmi.12919
PMID 25597841
MeSH Animals Aspergillus fumigatus / genetics Aspergillus fumigatus / pathogenicity* Aspergillus fumigatus / physiology* Aspergillus fumigatus / ultrastructure Biofilms / growth & development Cell Wall / metabolism Chitin / metabolism Computational Biology Disease Models, Animal Fungal Proteins / genetics Fungal Proteins / metabolism Gene Expression Regulation, Fungal* Glycerol / metabolism* Mice Mutation Osmolar Concentration* Phosphoric Monoester Hydrolases / genetics* Phosphoric Monoester Hydrolases / metabolism Signal Transduction beta-Glucans / metabolism
IF 3.418
Times Cited 29
Pathogenic microorganisms