RRC ID 40096
著者 Yoshimi A, Fujioka T, Mizutani O, Marui J, Hagiwara D, Abe K.
タイトル Mitogen-activated protein kinases MpkA and MpkB independently affect micafungin sensitivity in Aspergillus nidulans.
ジャーナル Biosci Biotechnol Biochem
Abstract The transcriptional regulation of the MAPK mpkA and cell wall-related genes in Aspergillus nidulans differs from that of their counterparts in Saccharomyces cerevisiae. The A. nidulans MAPK MpkB is putatively orthologous to the yeast MAPKs Kss1p and Fus3p. To investigate MpkB and its contribution to cell wall integrity in A. nidulans, we constructed mpkB-disruptant (mpkB∆) strains. We previously showed that mpkA∆ strains exhibited reduced colony growth and increased sensitivity to the β-1,3-glucan synthase inhibitor micafungin. Like mpkA∆ strains, mpkB∆ strains exhibited slight growth retardation and increased sensitivity to micafungin. Although MpkB-dependent signaling modulated the transcription of some cell wall-related genes, the sugar composition of cell wall fractions was similar among wild-type, mpkA∆, and mpkB∆ strains. To elucidate the relationship between MpkA and MpkB pathways, we compared conditional mutants of mpkB with those with mpkA deletion. Sensitivity testing suggested that MpkA and MpkB additively contribute to micafungin activity in A. nidulans.
巻・号 79(5)
ページ 836-44
公開日 2015-1-1
DOI 10.1080/09168451.2014.998619
PMID 25727969
MeSH Antifungal Agents / pharmacology Aspergillus nidulans / drug effects* Aspergillus nidulans / genetics Aspergillus nidulans / metabolism Cell Wall / chemistry Cell Wall / genetics Cell Wall / metabolism* Echinocandins / pharmacology* Fungal Proteins / genetics Fungal Proteins / metabolism Gene Expression Regulation, Fungal Lipopeptides / pharmacology* Micafungin Mitogen-Activated Protein Kinases / genetics Mitogen-Activated Protein Kinases / metabolism* Mutation Signal Transduction
IF 1.516
引用数 8
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED
リソース情報
病原微生物 NA