RRC ID 401
著者 Koshida S, Kishimoto Y, Ustumi H, Shimizu T, Furutani-Seiki M, Kondoh H, Takada S.
タイトル Integrinalpha5-dependent fibronectin accumulation for maintenance of somite boundaries in zebrafish embryos.
ジャーナル Dev Cell
Abstract Boundary formation and epithelialization are crucial processes in the morphological segmentation of vertebrate somites. By a genetic screening procedure with zebrafish, we identified two genes, integrinalpha5 (itga5) and fibronectin (fn), required for these processes. Fibronectin proteins accumulate at somite boundaries in accordance with epithelialization of the somites. Both Fibronectin accumulation and the epithelialization are dependent on itga5, which is expressed in the most medial part of somites. Although somite boundaries are initially formed, but not maintained, in the anterior trunk of the mutant embryos deficient in either gene, their maintenance is defective at all axial levels of embryos deficient for both of these genes. Therefore, Integrinalpha5-directed assembly of Fibronectin appears critical for epithelialization and boundary maintenance of somites. Furthermore, with an additional deficiency in ephrin-B2a, the segmental defect in itga5 or fn mutant embryos is expanded posteriorly, indicating that both Integrin-Fibronectin and Eph-Ephrin systems function cooperatively in maintaining somite boundaries.
巻・号 8(4)
ページ 587-98
公開日 2005-4-1
DOI 10.1016/j.devcel.2005.03.006
PII S1534-5807(05)00090-0
PMID 15809040
MeSH Animals Body Patterning* Cell Polarity Ephrin-B2 / genetics Ephrin-B2 / metabolism Epithelium / embryology Fibronectins / genetics Fibronectins / metabolism* Gene Expression Regulation, Developmental In Situ Hybridization Integrin alpha5 / genetics Integrin alpha5 / metabolism* Morphogenesis Oligonucleotides, Antisense / genetics Oligonucleotides, Antisense / metabolism Somites / cytology* Somites / physiology* Zebrafish / anatomy & histology Zebrafish / embryology* Zebrafish / genetics Zebrafish / metabolism
IF 10.092
引用数 136
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
ゼブラフィッシュ kt293 kt451 kt664 kt259 kt443?