論文 - 詳細
| RRC ID | 4015 |
|---|---|
| 著者 | Miao Y, Li KY, Li HY, Yao X, Jiang L. |
| タイトル | The vacuolar transport of aleurain-GFP and 2S albumin-GFP fusions is mediated by the same pre-vacuolar compartments in tobacco BY-2 and Arabidopsis suspension cultured cells. |
| ジャーナル | Plant J |
| Abstract |
Soluble proteins reach vacuoles because they contain vacuolar sorting determinants (VSDs) that are recognized by vacuolar sorting receptor (VSR) proteins. Pre-vacuolar compartments (PVCs), defined by VSRs and GFP-VSR reporters in tobacco BY-2 cells, are membrane-bound intermediate organelles that mediate protein traffic from the Golgi apparatus to the vacuole in plant cells. Multiple pathways have been demonstrated to be responsible for vacuolar transport of lytic enzymes and storage proteins to the lytic vacuole (LV) and the protein storage vacuole (PSV), respectively. However, the nature of PVCs for LV and PSV pathways remains unclear. Here, we used two fluorescent reporters, aleurain-GFP and 2S albumin-GFP, that represent traffic of lytic enzymes and storage proteins to LV and PSV, respectively, to study the PVC-mediated transport pathways via transient expression in suspension cultured cells. We demonstrated that the vacuolar transport of aleurain-GFP and 2S albumin-GFP was mediated by the same PVC populations in both tobacco BY-2 and Arabidopsis suspension cultured cells. These PVCs were defined by the seven GFP-AtVSR reporters. In wortmannin-treated cells, the vacuolated PVCs contained the mRFP-AtVSR reporter in their limiting membranes, whereas the soluble aleurain-GFP or 2S albumin-GFP remained in the lumen of the PVCs, indicating a possible in vivo relationship between receptor and cargo within PVCs. |
| 巻・号 | 56(5) |
| ページ | 824-39 |
| 公開日 | 2008-12-1 |
| DOI | 10.1111/j.1365-313X.2008.03645.x |
| PII | TPJ3645 |
| PMID | 18680561 |
| MeSH | 2S Albumins, Plant / genetics 2S Albumins, Plant / metabolism* Androstadienes / pharmacology Antigens, Plant / genetics Antigens, Plant / metabolism* Arabidopsis / drug effects Arabidopsis / genetics Arabidopsis / metabolism* Cells, Cultured Cysteine Endopeptidases / genetics Cysteine Endopeptidases / metabolism* Gene Expression Regulation, Plant Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Protein Transport Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Tobacco / drug effects Tobacco / genetics Tobacco / metabolism* Vacuoles / metabolism* Wortmannin |
| IF | 6.141 |
| 引用数 | 59 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda01250 |