Reference - Detail
| RRC ID | 4017 |
|---|---|
| Author | Kamada-Nobusada T, Hayashi M, Fukazawa M, Sakakibara H, Nishimura M. |
| Title | A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana. |
| Journal | Plant Cell Physiol |
| Abstract |
We characterized three Arabidopsis polyamine oxidase genes, AtPAO2, AtPAO3 and AtPAO4. Transient expression of these genes as monomeric red fluorescent protein fusion proteins in Arabidopsis root cells revealed that all are peroxisomal proteins. Quantitative analysis of their transcripts in various organs suggested that AtPAO4 is the major isoform in root peroxisomes. Analysis of recombinant AtPAO4 protein indicated that it is a flavoprotein that catalyzed the oxidative conversion of spermine to spermidine. AtPAO4-deficient mutants established by using T-DNA insertion and RNA interference techniques had markedly increased spermine and decreased spermidine levels in the roots. These results suggest that AtPAO4 is a root peroxisomal polyamine oxidase that participates in polyamine catabolism. Microarray analysis showed that AtPAO4 deficiency induced alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis. |
| Volume | 49(9) |
| Pages | 1272-82 |
| Published | 2008-9-1 |
| DOI | 10.1093/pcp/pcn114 |
| PII | pcn114 |
| PMID | 18703589 |
| MeSH | Arabidopsis / enzymology* Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* DNA, Bacterial / genetics Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Regulation, Plant Genes, Plant Mutagenesis, Insertional Oligonucleotide Array Sequence Analysis Oxidoreductases Acting on CH-NH Group Donors / genetics Oxidoreductases Acting on CH-NH Group Donors / metabolism* Peroxisomes / genetics Peroxisomes / metabolism* Plant Roots / enzymology Plant Roots / genetics RNA Interference RNA, Plant / genetics Recombinant Proteins / genetics Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Spermidine / metabolism Spermine / metabolism Substrate Specificity |
| IF | 4.062 |
| Times Cited | 104 |
| WOS Category | PLANT SCIENCES CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda07387 pda02195 pda04373 |