| RRC ID |
4045
|
| 著者 |
Ukitsu H, Kuromori T, Toyooka K, Goto Y, Matsuoka K, Sakuradani E, Shimizu S, Kamiya A, Imura Y, Yuguchi M, Wada T, Hirayama T, Shinozaki K.
|
| タイトル |
Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene.
|
| ジャーナル |
Plant Cell Physiol
|
| Abstract |
In transposon-tagged lines of Arabidopsis we found two new mutants, cof1-1 and cof1-2 (cuticular defect and organ fusion), that show the phenotype of wilting when grown in soil, organ fusion of rosette leaves and infertility. Toluidine blue testing and scanning electron microscopy observation revealed that these mutants had cuticular defects in the stems and adult leaves, but not in cotyledones. Transmission electron microscopy observation revealed thinner cuticle layers in the mutants, and cuticular materials interspersed between the two fused epidermal layers were observed in the mutant rosette leaves. These two mutants had a transposon insertion in the coding regions of WBC11, which was classified as a member of ABC transporter genes in Arabidopsis. WBC11 showed high sequence similarity to CER5 (also called WBC12), which was involved in cuticular lipid export. Gas chromatographic analysis revealed that C29 alkane extracted from the stem surface of cof1 mutants was reduced whereas C29 ketone was accumulated, which was different from the case of cer5 mutants. While cer5 mutants had fairly normal morphology, cof1 mutants had pleiotropic phenotypes so that COF1/WBC11 could have important roles different from those of CER5/WBC12. We also found that C29 alkane was accumulated in the intracellular extract of cof1 mutants, suggesting a function for WBC11 in the direct transport of lipid molecules. Pollen observation showed that mutant pollen grains were irregularly shaped. The function of COF1/WBC11 in lipid transport for the construction of cuticle layers and pollen coats for normal organ formation is discussed.
|
| 巻・号 |
48(11)
|
| ページ |
1524-33
|
| 公開日 |
2007-11-1
|
| DOI |
10.1093/pcp/pcm139
|
| PII |
pcm139
|
| PMID |
17971336
|
| MeSH |
ATP-Binding Cassette Transporters / genetics*
ATP-Binding Cassette Transporters / metabolism
ATP-Binding Cassette Transporters / physiology
Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis / ultrastructure
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Arabidopsis Proteins / physiology
Chromatography, Gas
Gene Expression Regulation, Plant
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Microscopy, Confocal
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Mutation*
Phylogeny
Plant Epidermis / genetics
Plant Epidermis / metabolism
Plant Epidermis / ultrastructure
Plant Leaves / genetics
Plant Leaves / metabolism
Plant Leaves / ultrastructure
Plants, Genetically Modified
Pollen / genetics
Pollen / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
4.062
|
| 引用数 |
61
|
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda07839 |
