論文 - 詳細
| RRC ID | 4050 |
|---|---|
| 著者 | Machida M, Takechi K, Sato H, Chung SJ, Kuroiwa H, Takio S, Seki M, Shinozaki K, Fujita T, Hasebe M, Takano H. |
| タイトル | Genes for the peptidoglycan synthesis pathway are essential for chloroplast division in moss. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
The general consensus is that a cyanobacterium phagocytosed by a host cell evolved into the plastids of red and green algae, land plants, and glaucophytes. In contrast to the plastids of glaucophytes, which retain a cyanobacterial-type peptidoglycan layer, no wall-like structures have been detected in plastids from other sources. Although the genome of Arabidopsis thaliana contains five genes that are essential for peptidoglycan synthesis, MurE, MurG, two genes for D-Ala-D-Ala ligase (Ddl), and the gene for translocase I (MraY), their functions have not been determined. We report that the moss Physcomitrella patens has nine homologous genes related to peptidoglycan biosynthesis: MurA, B, C, D, E, and F, Ddl, genes for the penicillin-binding protein Pbp, and dd-carboxypeptidase (Dac). Corroborating a computer prediction, analysis of the GFP fusion proteins with the N terminus of PpMurE or of PpPbp suggests that these proteins are located in the chloroplasts. Gene disruption of the PpMurE gene in P. patens resulted in the appearance of macrochloroplasts both in protonema and in leaf cells. Moreover, gene knockout of the P. patens Pbp gene showed inhibition of chloroplast division in this moss; however, no Pbp gene was found in A. thaliana. |
| 巻・号 | 103(17) |
| ページ | 6753-8 |
| 公開日 | 2006-4-25 |
| DOI | 10.1073/pnas.0510693103 |
| PII | 0510693103 |
| PMID | 16618924 |
| PMC | PMC1458953 |
| MeSH | Arabidopsis / genetics Arabidopsis / metabolism Base Sequence Bryopsida / genetics* Bryopsida / metabolism* Bryopsida / ultrastructure Chloroplasts / genetics* Chloroplasts / metabolism* DNA, Plant / genetics Evolution, Molecular Genes, Plant* Microscopy, Electron Molecular Sequence Data Peptidoglycan / biosynthesis* Peptidoglycan / genetics* |
| IF | 9.412 |
| 引用数 | 71 |
| WOS 分野 | MULTIDISCIPLINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pdp44800 pdp31890 |