| RRC ID |
4067
|
| Author |
An CI, Sawada A, Fukusaki E, Kobayashi A.
|
| Title |
A transient RNA interference assay system using Arabidopsis protoplasts.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.
|
| Volume |
67(12)
|
| Pages |
2674-7
|
| Published |
2003-12-1
|
| DOI |
10.1271/bbb.67.2674
|
| PMID |
14730153
|
| MeSH |
Arabidopsis / genetics*
Cells, Cultured
DNA Primers / genetics
Gene Expression Regulation, Plant / genetics*
Genes, Reporter / genetics*
Genetic Vectors / genetics*
Protoplasts / metabolism
RNA Interference*
RNA, Double-Stranded / genetics
Transfection
|
| IF |
1.516
|
| Times Cited |
21
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00008 |
