Reference - Detail
| RRC ID | 4067 |
|---|---|
| Author | An CI, Sawada A, Fukusaki E, Kobayashi A. |
| Title | A transient RNA interference assay system using Arabidopsis protoplasts. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana. |
| Volume | 67(12) |
| Pages | 2674-7 |
| Published | 2003-12-1 |
| DOI | 10.1271/bbb.67.2674 |
| PMID | 14730153 |
| MeSH | Arabidopsis / genetics* Cells, Cultured DNA Primers / genetics Gene Expression Regulation, Plant / genetics* Genes, Reporter / genetics* Genetic Vectors / genetics* Protoplasts / metabolism RNA Interference* RNA, Double-Stranded / genetics Transfection |
| IF | 1.516 |
| Times Cited | 21 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00008 |