| RRC ID |
40711
|
| 著者 |
Cho E, Park SN, Lim YK, Shin Y, Paek J, Hwang CH, Chang YH, Kook JK.
|
| タイトル |
Fusobacterium hwasookii sp. nov., Isolated from a Human Periodontitis Lesion.
|
| ジャーナル |
Curr Microbiol
|
| Abstract |
In this study, we classified the five strains (ChDC F128(T), ChDC F145, ChDC F174, ChDC F206, and ChDC F300) as a novel species of genus Fusobacterium by DNA-DNA hybridization and multi-locus phylogenetic analysis (MLPA), based on a single sequence (24,715 bp) of 22 concatenated housekeeping genes, with morphological and chemotaxonomic characteristics. DNA-DNA hybridization data showed that the values of genomic relatedness between ChDC F128(T) and each of the other novel strains were ranged from 79.0 to 82.6 %, while those of genomic relatedness between ChDC F128(T) and type strain of each of subspecies of F. nucleatum or Fusobacterium periodonticum were ranged from 40.9 to 54.4 %. MLPA revealed that the 5 strains were clustered as one group and clearly discriminated with F. nucleatum and F. periodonticum with 100 % bootstrap value. The DNA G+C content of the five novel strains were ranged from 26.9 to 27.0 mol%. The cellular fatty acid analysis of clinical isolates and type strains revealed C14:0, C16:0, and cis-9 C16:1 as the major fatty acids. The cell wall peptidoglycan of the 5 strains was comprised of meso-lanthionine. These results show that the 5 strains are novel species and belong to the genus Fusobacterium. Strain ChDC F128(T) (=KCOM 1249(T) = KCTC 5108(T) = JCM 30218(T)) is suggested to be the type strain of a novel species of genus Fusobacterium, for which the name Fusobacterium hwasookii sp. nov. is proposed.
|
| 巻・号 |
70(2)
|
| ページ |
169-75
|
| 公開日 |
2015-2-1
|
| DOI |
10.1007/s00284-014-0692-7
|
| PMID |
25257648
|
| MeSH |
Base Composition
DNA, Bacterial
Fusobacterium* / classification
Fusobacterium* / genetics
Fusobacterium* / metabolism
Fusobacterium Infections / microbiology*
Genes, Essential
Humans
Multilocus Sequence Typing
Periodontitis / microbiology*
Phylogeny
|
| IF |
1.746
|
| 引用数 |
14
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 30218
JCM 30219
JCM 30221
JCM 30222 |
