論文 - 詳細
| RRC ID | 40721 |
|---|---|
| 著者 | Xing J, Liu C, Zhang Y, He H, Zhou Y, Li L, Zhao J, Liu S, Wang X, Xiang W. |
| タイトル | Sphaerisporangium dianthi sp. nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
A novel actinomycete, designated strain NEAU-CY18(T), was isolated from the root of a Chinese medicinal plant Dianthus chinensis L and subjected to a polyphasic taxonomic study. The novel strain was found to develop spherical sporangia with non-motile spores on aerial mycelium. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The whole-cell sugars were identified as madurose, mannose, ribose, galactose and glucose. The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were identified as MK-9(H4), MK-9(H2) and MK-9(H6). The major fatty acids were identified as C17:0 10-methyl, iso-C16:0 and C16:0. EzTaxon-e analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Sphaerisporangium and was most closely related to Sphaerisporangium cinnabarinum JCM 3291(T) (98.9 %) and Sphaerisporangium melleum JCM 13064(T) (98.3 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-CY18(T) forms a monophyletic clade with S. cinnabarinum JCM 3291(T), an association that was supported by a bootstrap value of 97 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. Comparisons of some phenotypic properties and low DNA-DNA relatedness values enabled the strain to be differentiated from S. cinnabarinum JCM 3291(T) and S. melleum JCM 13064(T). Therefore, it is concluded that strain NEAU-CY18(T) represents a novel Sphaerisporangium species, for which the name Sphaerisporangium dianthi sp. nov. is proposed. The type strain is NEAU-CY18(T) ( = CGMCC 4.7132(T) = DSM 46736(T)). |
| 巻・号 | 107(1) |
| ページ | 9-14 |
| 公開日 | 2015-1-1 |
| DOI | 10.1007/s10482-014-0298-x |
| PMID | 25294725 |
| MeSH | Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Bacterial Typing Techniques Carbohydrates / analysis Cell Wall / chemistry Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Dianthus / microbiology* Endophytes / classification* Endophytes / genetics Endophytes / isolation & purification* Fatty Acids / analysis Locomotion Molecular Sequence Data Nucleic Acid Hybridization Peptidoglycan / analysis Phylogeny Plant Roots / microbiology Plants, Medicinal / microbiology RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Vitamin K 2 / analysis |
| IF | 2.674 |
| 引用数 | 5 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 3291 JCM 13064 |