Reference - Detail
| RRC ID | 40731 |
|---|---|
| Author | Su X, Liu Y, Hashmi MZ, Hu J, Ding L, Wu M, Shen C. |
| Title | Rhodococcus biphenylivorans sp. nov., a polychlorinated biphenyl-degrading bacterium. |
| Journal | Antonie Van Leeuwenhoek |
| Abstract |
A Gram-positive, aerobic, non-motile and rod-coccus shaped novel actinobacterial strain, designated as TG9(T), was isolated from a polychlorinated biphenyl (PCB)-contaminated sediment in Taizhou city, Zhejiang province, eastern China. The isolate was observed to grow at 10-45 °C (optimum 28-32 °C), pH 5.0-11.0 (optimum pH 7.0-8.0) and with 0-9.0 % (w/v) NaCl (optimum 0-3.0 %). Comparison of the 16S rRNA gene sequences of strain TG9(T) and other members of the genus Rhodococcus showed that strain TG9(T) shared highest similarities with Rhodococcus pyridinivorans DSM 44555(T) (99.4 %), R. rhodochrous DSM 43241(T) (99.2 %), R. gordoniae DSM 44689(T) (99.2 %) and R. artemisiae DSM 45380(T) (98.2 %). However, low levels of DNA-DNA relatedness (15-48 %), which are below the 70 % limit for prokaryotic species identification, were obtained by DNA-DNA hybridization. Strain TG9(T) was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and arabinose and galactose in the whole-cell hydrolysate. Mycolic acids were found to be present. The major fatty acids were identified as C16:0, C16:1 ω7c and/or iso-C15:0 2-OH, 10-methyl C18:0 and C18:1 ω9c. The only menaquinone detected was MK-8 (H2). The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, glycolipid and traces of some unknown lipids. The genomic DNA G+C content of strain TG9(T) was determined to be 62.8 %. The combined phenotypic and genotypic data show that the strain represents a novel species of the genus Rhodococcus for which the name Rhodococcus biphenylivorans sp. nov. is proposed, with the type strain TG9(T) (=CGMCC 1.12975(T) = KCTC 29673(T) = MCCC 1K00286(T)). |
| Volume | 107(1) |
| Pages | 55-63 |
| Published | 2015-1-1 |
| DOI | 10.1007/s10482-014-0303-4 |
| PMID | 25315102 |
| MeSH | Aerobiosis Bacterial Typing Techniques Base Composition Biotransformation Cell Wall / chemistry China Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / analysis Fatty Acids / analysis Hydrogen-Ion Concentration Locomotion Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / analysis Phylogeny Polychlorinated Biphenyls / metabolism* RNA, Ribosomal, 16S / genetics Rhodococcus / classification* Rhodococcus / genetics Rhodococcus / isolation & purification* Rhodococcus / metabolism Sequence Analysis, DNA Sodium Chloride / metabolism Soil Pollutants / metabolism Temperature Vitamin K 2 / analysis |
| IF | 2.674 |
| Times Cited | 10 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 10940 JCM 3202 JCM 12658 |