RRC ID 40799
Author Won KH, Kook M, Yi TH.
Title Pedobacter bambusae sp. nov., isolated from soil of a bamboo plantation.
Journal Antonie Van Leeuwenhoek
Abstract An ivory white, rod-shaped, non-motile, non-spore forming, Gram-staining-negative, and aerobic bacterium designated THG-G118T, was isolated from a soil sample collected in a bamboo plantation in Seoul, Republic of Korea. The 16S rRNA gene sequence analysis revealed a clear affiliation of strain THG-G118T with the genus Pedobacter. Strain THG-G118T was found to show the closest phylogenetic relationship with Pedobacter metabolipauper WB2.3-71T with 16S rRNA gene sequence similarity of 97.3 %. The major cellular fatty acids of strain THG-G118T were identified as C16:0, C18:0, iso-C15:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and the predominant menaquinone as menaquinone MK-7. The major polar lipids were found to be phosphatidylethanolamine (PE), unidentified phospholipid and unidentified aminophospholipids. Strain THG-G118T was found to contain ceramide phosphorylethanolamine 2 (CerPE-2) and dihydrosphingosine as the major ceramide. The G+C content of the genomic DNA was found to be 41.7 mol%. DNA-DNA hybridization experiments revealed a low level of DNA-DNA relatedness (<42.5 %) between strain THG-G118T and its closest relatives. The phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-G118T could be clearly distinguished from the members of the genus Pedobacter. Based on the results of a polyphasic study, a new species, Pedobacter bambusae sp. nov., is proposed. The type strain is THG-G118T (=KACC 17544T = JCM 19364T).
Volume 107(2)
Pages 565-73
Published 2015-2-1
DOI 10.1007/s10482-014-0352-8
PMID 25520261
MeSH Aerobiosis Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Korea Molecular Sequence Data Nucleic Acid Hybridization Pedobacter / classification* Pedobacter / genetics Pedobacter / isolation & purification* Pedobacter / physiology Phospholipids / analysis Phylogeny Poaceae / growth & development RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Vitamin K 2 / analysis
IF 2.674
Times Cited 6
General Microbes JCM 19364