RRC ID 408
Author Miskey C, Izsvák Z, Kawakami K, Ivics Z.
Title DNA transposons in vertebrate functional genomics.
Journal Cell. Mol. Life Sci.
Abstract Genome sequences of many model organisms of developmental or agricultural importance are becoming available. The tremendous amount of sequence data is fuelling the next phases of challenging research: annotating all genes with functional information, and devising new ways for the experimental manipulation of vertebrate genomes. Transposable elements are known to be efficient carriers of foreign DNA into cells. Notably, members of the Tc1/mariner and the hAT transposon families retain their high transpositional activities in species other than their hosts. Indeed, several of these elements have been successfully used for transgenesis and insertional mutagenesis, expanding our abilities in genome manipulations in vertebrate model organisms. Transposon-based genetic tools can help scientists to understand mechanisms of embryonic development and pathogenesis, and will likely contribute to successful human gene therapy. We discuss the possibilities of transposon-based techniques in functional genomics, and review the latest results achieved by the most active DNA transposons in vertebrates. We put emphasis on the evolution and regulation of members of the best-characterized and most widely used Tc1/mariner family.
Volume 62(6)
Pages 629-41
Published 2005-3
DOI 10.1007/s00018-004-4232-7
PMID 15770416
MeSH Animals DNA Repair DNA Transposable Elements* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Evolution, Molecular Genomics* Humans Mutagenesis, Insertional Transposases / metabolism
IF 6.721
Times Cited 66
Zebrafish Review