論文 - 詳細
| RRC ID | 40817 |
|---|---|
| 著者 | Nakayama M, Shimatani K, Ozawa T, Shigemune N, Tomiyama D, Yui K, Katsuki M, Ikeda K, Nonaka A, Miyamoto T. |
| タイトル | Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Catechins are a class of polyphenols and have high anti-bacterial activity against various microorganisms. Here, we report the mechanism for antibacterial activity of epigallocatechin gallate (EGCg) against Gram-positive bacteria Bacillus subtilis, which is highly sensitive to EGCg. Transmission electron microscope analysis revealed that deposits containing EGCg were found throughout the cell envelope from the outermost surface to the outer surface of cytoplasmic membrane. Aggregating forms of proteins and EGCg were identified as spots that disappeared or showed markedly decreased intensity after the treatment with EGCg compared to the control by two-dimensional electrophoresis. Among the identified proteins included 4 cell surface proteins, such as oligopeptide ABC transporter binding lipoprotein, glucose phosphotransferase system transporter protein, phosphate ABC transporter substrate-binding protein, and penicillin-binding protein 5. Observations of glucose uptake of cells and cell shape B. subtilis after the treatment with EGCg suggested that EGCg inhibits the major functions of these proteins, leading to growth inhibition of B. subtilis. |
| 巻・号 | 79(5) |
| ページ | 845-54 |
| 公開日 | 2015-1-1 |
| DOI | 10.1080/09168451.2014.993356 |
| PMID | 25559894 |
| MeSH | Amino Acid Sequence Anti-Bacterial Agents / pharmacology* Bacillus subtilis / drug effects* Bacillus subtilis / metabolism Bacillus subtilis / physiology Bacterial Proteins / antagonists & inhibitors Bacterial Proteins / chemistry Bacterial Proteins / metabolism* Carrier Proteins / chemistry Carrier Proteins / metabolism Catechin / analogs & derivatives* Catechin / chemistry Catechin / metabolism Catechin / pharmacology Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Glucose / metabolism Lipoproteins / chemistry Lipoproteins / metabolism Molecular Docking Simulation Molecular Sequence Data Oxygen / metabolism Spores, Bacterial / drug effects |
| IF | 1.516 |
| 引用数 | 32 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1465 |