RRC ID 40827
Author Wang HF, Zhang YG, Li L, Liu WH, Hozzein WN, Chen JY, Guo JW, Zhang YM, Li WJ.
Title Okibacterium endophyticum sp. nov., a novel endophytic actinobacterium isolated from roots of Salsola affinis C. A. Mey.
Journal Antonie Van Leeuwenhoek
Abstract A white bacterial strain, designated EGI 650022(T), was isolated from the roots of Salsola affinis C. A. Mey, collected from Urumqi City, Xinjiang, north-western China. The strain was found to be aerobic, Gram-stain positive, oxidase-positive and catalase-positive. Cells were non-motile and irregular rods. Growth occurred at NaCl concentrations between 0 and 7 % (w/v), temperatures between 5 and 45 °C, and pH 6.0-9.0. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain EGI 650022(T) belongs to a clade with the genera Okibacterium and Plantibacter in the family Microbacteriaceae. The novel strain EGI 650022(T) showed highest levels of 16S rRNA gene sequence similarity with members of the genera Okibacterium and Plantibacter (97.2-98.0 %). The cell-wall peptidoglycan contained glutamate, homoserine, glycine, alanine and lysine. The predominant menaquinones (MKs) were MK-11, MK-12 and MK-12 (H4). The polar lipid pattern comprised phosphatidylglycerol, diphosphatidylglycerol, two unknown glycolipids and two unknown phospholipids. The major fatty acids were anteiso-C15:0 and anteiso-C17:0. The DNA G+C content was 66.0 mol%. The DNA-DNA relatedness values of strain EGI 650022(T) with Okibacterium fritillariae DSM 12584(T), Plantibacter flavus DSM 14012(T) and Plantibacter auratus DSM 19586(T) were 39.7, 19.7 and 22.0 %. Based on phylogenetic, phenotypic, chemotaxonomic and DNA-DNA hybridization data, strain EGI 650022(T) is considered to represent a novel species of the genus Okibacterium, for which the name Okibacterium endophyticum sp. nov. is proposed; the type strain is EGI 650022(T) (=JCM 30086(T) = KCTC 29492(T)).
Volume 107(3)
Pages 835-43
Published 2015-3
DOI 10.1007/s10482-014-0376-0
PMID 25566956
MeSH Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Aerobiosis Base Composition Catalase / analysis Cell Wall / chemistry China Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Endophytes / classification* Endophytes / genetics Endophytes / isolation & purification* Fatty Acids / analysis Glutamic Acid / analysis Glycolipids / analysis Hydrogen-Ion Concentration Molecular Sequence Data Nucleic Acid Hybridization Oxidoreductases / analysis Peptidoglycan / analysis Phospholipids / analysis Phylogeny Plant Roots / microbiology RNA, Ribosomal, 16S / genetics Salsola / microbiology* Sequence Analysis, DNA Sodium Chloride / metabolism Temperature Vitamin K 2 / analysis
IF 1.588
Times Cited 3
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 30086 JCM 12284