論文 - 詳細
| RRC ID | 40882 |
|---|---|
| 著者 | Lee JM, Kim YR, Kim JK, Jeong GT, Ha JC, Kong IS. |
| タイトル | Characterization of salt-tolerant β-glucosidase with increased thermostability under high salinity conditions from Bacillus sp. SJ-10 isolated from jeotgal, a traditional Korean fermented seafood. |
| ジャーナル | Bioprocess Biosyst Eng |
| Abstract |
The β-glucosidase gene, bglC, was cloned from Bacillus sp. SJ-10 isolated from the squid jeotgal. Recombinant BglC protein overexpression was induced in Escherichia coli. The optimal pH and temperature of the enzyme, using p-nitrophenyl-β-D-glucopyranoside (pNPβGlc) as a substrate, were pH 6 and 40 °C, respectively. Enzymatic activity increased by 3.3- and 3.5-fold in the presence of 15% NaCl and KCl, respectively. Furthermore, enzyme thermostability improved in the presence of NaCl or KCl. At 45 °C in the presence of salts, the enzyme was stable for 2 h and maintained 80% activity. In the absence of salts, BglC completely lost activity after 110 min at 45 °C. Comparison of the kinetic parameters at various salt concentrations revealed that BglC had approximately 1.5- and 1.2-fold higher affinity and hydrolyzed pNPβGlc 1.9- and 2.1-fold faster in the presence of 15% NaCl and KCl, respectively. Additionally, the Gibb's free energy for denaturation was higher in the presence of 15% salt than in the absence of salt at 45 and 50 °C. Since enzymatic activity and thermostability were enhanced under high salinity conditions, BglC is an ideal salt-tolerant enzyme for further research and industrial applications. |
| 巻・号 | 38(7) |
| ページ | 1335-46 |
| 公開日 | 2015-7-1 |
| DOI | 10.1007/s00449-015-1375-x |
| PMID | 25682105 |
| MeSH | Bacillus / metabolism* Cloning, Molecular Enzyme Stability Fermentation* Hot Temperature Kinetics Republic of Korea Salinity* Seafood* Substrate Specificity beta-Glucosidase / genetics beta-Glucosidase / metabolism* |
| IF | 2.419 |
| 引用数 | 16 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENGINEERING, CHEMICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 15709 |