論文 - 詳細
| RRC ID | 40901 |
|---|---|
| 著者 | Minegishi H, Echigo A, Shimane Y, Kamekura M, Itoh T, Ohkuma M, Usami R. |
| タイトル | Halococcus agarilyticus sp. nov., an agar-degrading haloarchaeon isolated from commercial salt. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
Two agar-degrading halophilic archaeal strains, 62 E(T) and 197 A, were isolated from commercial salt samples. Cells were non-motile cocci, approximately 1.2-2.0 µm in diameter and stained Gram-negative. Colonies were pink-pigmented. Strain 62 E(T) was able to grow with 24-30% (w/v) NaCl (optimum, 27%), at pH 6.5-8.5 (optimum, pH 7.5) and at 22-47 °C (optimum, 42 °C). The 16S rRNA gene sequences of strains 62 E(T) and 197 A were identical, and the level of DNA-DNA relatedness between them was 90 and 90% (reciprocally). The closest relative was Halococcus saccharolyticus JCM 8878(T) with 99.7% similarity in 16S rRNA orthologous gene sequences, followed by Halococcus salifodinae JCM 9578(T) (99.6%), while similarities with other species of the genus Halococcus were equal to or lower than 95.1%. The rpoB' gene tree strongly supported that the two strains were members of the genus Halococcus . Mean DNA-DNA relatedness between strain 62 E(T) and H. saccharolyticus JCM 8878(T) and H. salifodinae JCM 9578(T) was 46 and 44%, respectively. The major polar lipids were archaeol derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, derived from both C20C20 and C20C25 archaeol, and sulfated diglycosyl archaeol-1. Several unidentified glycolipids were present. Based on the phenotypic and phylogenetic analyses, the isolates are considered to represent a novel species of the genus Halococcus , for which the name Halococcus agarilyticus sp. nov. is proposed. The type strain is 62 E(T) ( = JCM 19592(T) =KCTC 4143(T)). |
| 巻・号 | 65(Pt 5) |
| ページ | 1634-1639 |
| 公開日 | 2015-5-1 |
| DOI | 10.1099/ijs.0.000151 |
| PMID | 25721723 |
| MeSH | Agar Base Composition DNA, Archaeal / genetics Genes, Archaeal Glycolipids / chemistry Halococcus / classification* Halococcus / genetics Halococcus / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phosphatidylglycerols / chemistry Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride* |
| IF | 2.415 |
| 引用数 | 3 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 8876 JCM 8878 JCM 9578 JCM 12289 JCM 12892 JCM 13552 JCM 13587 JCM 19592 |