RRC ID 40910
著者 Modesto M, Michelini S, Stefanini I, Sandri C, Spiezio C, Pisi A, Filippini G, Biavati B, Mattarelli P.
タイトル Bifidobacterium lemurum sp. nov., from faeces of the ring-tailed lemur (Lemur catta).
ジャーナル Int J Syst Evol Microbiol
Abstract Four Gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase-positive bacterial strains were isolated from a faecal sample of a 5-year-old ring-tailed lemur (Lemur catta). The strains showed a peculiar morphology, resembling a small coiled snake, a ring shape, or forming a little 'Y' shape. The isolated strains appeared identical, and LMC 13T was chosen as a representative strain and characterized further. Strain LMC 13T showed an A3β peptidoglycan type, similar to that found in Bifidobacterium longum. The DNA base composition was 57.2 mol% G+C. Almost-complete 16S rRNA, hsp60, rpoB, dnaJ, dnaG, purF, clpC and rpoC gene sequences were obtained, and phylogenetic relationships were determined. Comparative analysis of 16S rRNA gene sequences showed that strain LMC 13T showed the highest similarity to B. longum subsp. suis ATCC 27533T (96.65 %) and Bifidobacterium saguini DSM 23967T (96.64 %). Strain LMC 13T was located in an actinobacterial cluster and was more closely related to the genus Bifidobacteriumthan to other genera in the Bifidobacteriaceae. On the basis of these results, strain LMC 13T represents a novel species within the genus Bifidobacterium, for which the name Bifidobacterium lemurum sp. nov. is proposed; the type strain is LMC 13T ( = DSM 28807T = JCM 30168T).
巻・号 65(Pt 6)
ページ 1726-1734
公開日 2015-6-1
DOI 10.1099/ijs.0.000162
PMID 25736415
MeSH Animals Bacterial Typing Techniques Base Composition Bifidobacterium / classification* Bifidobacterium / genetics Bifidobacterium / isolation & purification DNA, Bacterial / genetics Feces / microbiology Genes, Bacterial Lemur / microbiology* Molecular Sequence Data Multilocus Sequence Typing Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.415
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30168