RRC ID 40916
Author Kodani S, Komaki H, Suzuki M, Hemmi H, Ohnishi-Kameyama M.
Title Isolation and structure determination of new siderophore albachelin from Amycolatopsis alba.
Journal Biometals
Abstract A new siderophore named albachelin was isolated from iron deficient culture of Amycolatopsis alba. The planar structure of albachelin was elucidated by the combination of ESI-MS/MS experiment and NMR spectroscopic analyses of the gallium (III) complex. The structure of albachelin was determined to be a linear peptide consisting of 6 mol of amino acids including 3 mol of serine, one mol each of N-α-acethyl-N-δ-hydroxy-N-δ-formylornithine, N-α-methyl-N-δ-hydroxyornithine, and cyclic N-hydroxyornithine. The stereochemistries of amino acids constituting albachelin were analyzed by applying modified Marfey method to the hydrolysate of albachelin. Based on bioinformatics, we deduced and discussed the possible biosynthetic gene cluster involved in albachelin biosynthesis from the genome sequence of A. alba. By prediction of substrates for adenylation domains, a non-ribosomal peptide biosynthetase gene (AMYAL_RS0130210) was proposed to be the main biosynthetic gene for albachelin biosynthesis. The related genes including transporter for siderophore were found near the NRPS gene as a gene cluster.
Volume 28(2)
Pages 381-9
Published 2015-4
DOI 10.1007/s10534-015-9842-z
PMID 25749409
MeSH Actinomycetales / chemistry Actinomycetales / genetics* Actinomycetales / metabolism Bacterial Proteins / genetics Biosynthetic Pathways Gallium / chemistry Genes, Bacterial Magnetic Resonance Spectroscopy Molecular Structure Multigene Family Siderophores / biosynthesis Siderophores / chemistry* Siderophores / isolation & purification Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry
IF 2.478
Times Cited 3
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 10030