RRC ID 40933
著者 Quadri SR, Tian XP, Zhang J, Li J, Nie GX, Tang SK, Al Ruwaili J, Agsar D, Li WJ, Dastager SG.
タイトル Nonomuraea indica sp. nov., novel actinomycetes isolated from lime-stone open pit mine, India.
ジャーナル J Antibiot (Tokyo)
Abstract A Gram-positive, aerobic, nonmotile actinomycete strain designated DRQ-2(T) was isolated from the soil sample collected from lime-stone open pit mine from the Gulbarga region, Karnataka province, India. Strain DRQ-2(T) was identified as a member of the genus Nonomuraea by a polyphasic approach. Strain DRQ-2(T) could be differentiated from other members of the genus Nonomuraea on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain DRQ-2(T) showed highest sequence similarity to Nonomuraea muscovyensis DSM 45913(T) (99.1%), N. salmonea DSM 43678(T) (98.2%) and N. maheshkhaliensis JCM 13929(T) with 98.0%, respectively. Chemotaxonomic properties showing predominant menaquinones of MK-9 (H4), MK-9(H2) and MK-9(H6), major polar lipids comprised diphosphatidylglycerol, phosphatidylmono methyl ethanolamine (PME), phosphatidylethanolamine (PE), hydroxy-PME (OH-PME), hydroxy PE (OH-PEE), phosphatidylglycerol (PG), ninhydrin-positive phosphoglycolipid and unknown phospholipid, fatty acids with major amounts of i-C16:0, ai-C15:0 and ai-C17:0 supported allocation of the strain to the genus Nonomuraea. Results of DNA-DNA hybridization and physiological tests allowed genotypic and phenotypic differentiation of strain DRQ-2(T) from closely related species. The genomic DNA G+C content of the organism was 72.5 mol%. On the basis of phenotypic, chemotypic and molecular characteristics, strain DRQ-2(T) represents a novel species of the genus Nonomuraea, for which the name N. indica sp. nov. is proposed, with type strain DRQ-2(T) (=NCIM 5480(T)= CCTCC AA 209050(T)).
巻・号 68(8)
ページ 491-5
公開日 2015-8-1
DOI 10.1038/ja.2015.24
PII ja201524
PMID 25783226
MeSH Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Actinobacteria / physiology Aerobiosis Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Glycolipids / analysis India Microscopy, Electron, Scanning Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology Vitamin K 2 / analysis
IF 2.668
引用数 7
WOS 分野 PHARMACOLOGY & PHARMACY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY IMMUNOLOGY
リソース情報
一般微生物 JCM 13929