RRC ID |
41000
|
著者 |
Tanimura A, Kikukawa M, Yamaguchi S, Kishino S, Ogawa J, Shima J.
|
タイトル |
Direct ethanol production from starch using a natural isolate, Scheffersomyces shehatae: Toward consolidated bioprocessing.
|
ジャーナル |
Sci Rep
|
Abstract |
Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a one-step process, is a promising strategy for cost-effective ethanol production from starchy biomass. To gain insights into starch-based ethanol production using CBP, an extensive screening was undertaken to identify naturally occurring yeasts that produce ethanol without the addition of any amylases. Three yeast strains were capable of producing a significant amount of ethanol. Quantitative assays revealed that Scheffersomyces shehatae JCM 18690 was the strain showing the highest ethanol production ability. This strain was able to utilize starch directly, and the ethanol concentration reached 9.21 g/L. We attribute the ethanol-producing ability of this strain to the high levels of glucoamylase activity, fermentation potential and ethanol stress tolerance. This study strongly suggests the possibility of starch-based ethanol production by consolidated bioprocessing using natural yeasts such as S. shehatae JCM 18690.
|
巻・号 |
5
|
ページ |
9593
|
公開日 |
2015-4-22
|
DOI |
10.1038/srep09593
|
PII |
srep09593
|
PMID |
25901788
|
PMC |
PMC5386104
|
MeSH |
Biomass
Ethanol / metabolism*
Fermentation
Glucan 1,4-alpha-Glucosidase / metabolism
Saccharomycetales / enzymology
Saccharomycetales / metabolism*
Starch / metabolism
alpha-Amylases / metabolism
|
IF |
3.998
|
引用数 |
24
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 18690 |