RRC ID 41000
著者 Tanimura A, Kikukawa M, Yamaguchi S, Kishino S, Ogawa J, Shima J.
タイトル Direct ethanol production from starch using a natural isolate, Scheffersomyces shehatae: Toward consolidated bioprocessing.
ジャーナル Sci Rep
Abstract Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a one-step process, is a promising strategy for cost-effective ethanol production from starchy biomass. To gain insights into starch-based ethanol production using CBP, an extensive screening was undertaken to identify naturally occurring yeasts that produce ethanol without the addition of any amylases. Three yeast strains were capable of producing a significant amount of ethanol. Quantitative assays revealed that Scheffersomyces shehatae JCM 18690 was the strain showing the highest ethanol production ability. This strain was able to utilize starch directly, and the ethanol concentration reached 9.21 g/L. We attribute the ethanol-producing ability of this strain to the high levels of glucoamylase activity, fermentation potential and ethanol stress tolerance. This study strongly suggests the possibility of starch-based ethanol production by consolidated bioprocessing using natural yeasts such as S. shehatae JCM 18690.
巻・号 5
ページ 9593
公開日 2015-4-22
DOI 10.1038/srep09593
PII srep09593
PMID 25901788
PMC PMC5386104
MeSH Biomass Ethanol / metabolism* Fermentation Glucan 1,4-alpha-Glucosidase / metabolism Saccharomycetales / enzymology Saccharomycetales / metabolism* Starch / metabolism alpha-Amylases / metabolism
IF 3.998
引用数 24
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
一般微生物 JCM 18690