RRC ID 41059
著者 Li C, Wang H, Jin P, Zheng W, Chu L, Liu C, Li J, Xiang W, Wang X.
タイトル Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta).
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel endophytic actinomycete, strain NEAU-TX1-15(T), was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15(T). Morphological and chemotaxonomic properties of strain NEAU-TX1-15(T) are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15(T) was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H6) and MK-9(H8). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C16:0. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686(T). However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15(T) could also be differentiated from A. yoronensis NBRC 103686(T) and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (>98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA-DNA hybridization value, indicated that strain NEAU-TX1-15(T) represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15(T) (=CGMCC 4.7200(T) = JCM 30340(T)).
巻・号 108(2)
ページ 453-9
公開日 2015-8-1
DOI 10.1007/s10482-015-0498-z
PMID 26033369
MeSH Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Actinobacteria / physiology Bacterial Typing Techniques Bryophyta / microbiology* Cell Wall / chemistry China Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / analysis Endophytes / classification* Endophytes / genetics Endophytes / isolation & purification* Endophytes / physiology Fatty Acids / analysis Microscopy, Electron, Scanning Molecular Sequence Data Nucleic Acid Hybridization Peptidoglycan / analysis Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Spores, Bacterial / cytology Vitamin K 2 / analysis
IF 2.674
引用数 1
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30340