RRC ID 41079
著者 Han TY, Tong XM, Wang YW, Wang HM, Chen XR, Kong DL, Guo X, Ruan ZY.
タイトル Paenibacillus populi sp. nov., a novel bacterium isolated from the rhizosphere of Populus alba.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel aerobic bacterium, designated strain LAM0705(T), was isolated from the rhizosphere of Populus alba in the Peking University Third Hospital. Cells of strain LAM0705(T) were observed to be Gram-stain positive, motile, spore-forming and rod-shaped. The optimal temperature and pH for growth were found to be 30 °C and pH 7.5, respectively. Strain LAM0705(T) was found to be able to grow in the presence 0-5 % NaCl (w/v) (optimum 1.0 %). The major fatty acids of strain LAM0705(T) were identified as anteiso-C15:0, C16:0 and iso-C16:0. The dominant polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The cell wall peptidoglycan of strain LAM0705(T) was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The G+C content of genomic DNA was found to be 48 mol% when determined by the T m method. The 16S rRNA gene sequence similarity analysis indicated that strain LAM0705(T) is closely related to Paenibacillus agaridevorans DSM 1355(T) and Paenibacillus thailandensis KCTC 13043(T) with 97.8 and 96.1 % sequence similarity, respectively. The DNA-DNA hybridization value between strain LAM0705(T) and P. agaridevorans DSM 1355(T) was 47 ± 0.8 %. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0705(T) is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus populi sp. nov. is proposed. The type strain is LAM0705(T) (=ACCC 06427(T) = JCM 19843(T)).
巻・号 108(3)
ページ 659-66
公開日 2015-9-1
DOI 10.1007/s10482-015-0521-4
PMID 26133115
MeSH Aerobiosis Bacterial Typing Techniques Base Composition Cell Wall / chemistry China Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / analysis Fatty Acids / analysis Hydrogen-Ion Concentration Locomotion Molecular Sequence Data Nucleic Acid Hybridization Paenibacillus / classification* Paenibacillus / genetics Paenibacillus / isolation & purification* Paenibacillus / physiology Peptidoglycan / analysis Phospholipids / analysis Phylogeny Populus* RNA, Ribosomal, 16S / genetics Rhizosphere* Sequence Analysis, DNA Sodium Chloride / metabolism Soil Microbiology* Spores, Bacterial / cytology Temperature Vitamin K 2 / analysis
IF 2.674
引用数 6
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19843