RRC ID |
41187
|
著者 |
Sugahara H, Odamaki T, Fukuda S, Kato T, Xiao JZ, Abe F, Kikuchi J, Ohno H.
|
タイトル |
Probiotic Bifidobacterium longum alters gut luminal metabolism through modification of the gut microbial community.
|
ジャーナル |
Sci Rep
|
Abstract |
Probiotics are well known as health-promoting agents that modulate intestinal microbiota. However, the molecular mechanisms underlying this effect remain unclear. Using gnotobiotic mice harboring 15 strains of predominant human gut-derived microbiota (HGM), we investigated the effects of Bifidobacterium longum BB536 (BB536-HGM) supplementation on the gut luminal metabolism. Nuclear magnetic resonance (NMR)-based metabolomics showed significantly increased fecal levels of pimelate, a precursor of biotin, and butyrate in the BB536-HGM group. In addition, the bioassay revealed significantly elevated fecal levels of biotin in the BB536-HGM group. Metatranscriptomic analysis of fecal microbiota followed by an in vitro bioassay indicated that the elevated biotin level was due to an alteration in metabolism related to biotin synthesis by Bacteroides caccae in this mouse model. Furthermore, the proportion of Eubacterium rectale, a butyrate producer, was significantly higher in the BB536-HGM group than in the group without B. longum BB536 supplementation. Our findings help to elucidate the molecular basis underlying the effect of B. longum BB536 on the gut luminal metabolism through its interactions with the microbial community.
|
巻・号 |
5
|
ページ |
13548
|
公開日 |
2015-8-28
|
DOI |
10.1038/srep13548
|
PII |
srep13548
|
PMID |
26315217
|
PMC |
PMC4552000
|
MeSH |
Animals
Bifidobacterium / drug effects
Bifidobacterium / genetics
Bifidobacterium / metabolism*
Biotin / metabolism
Feces / microbiology
Gastrointestinal Tract / drug effects
Gastrointestinal Tract / microbiology*
Gene Expression Regulation / drug effects
Germ-Free Life
Humans
Magnetic Resonance Spectroscopy
Metabolome / drug effects
Metabolomics
Mice
Microbiota / drug effects*
Probiotics / pharmacology*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Solubility
Species Specificity
Water
|
IF |
3.998
|
引用数 |
63
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
一般微生物 |
JCM 15632
JCM 5828
JCM 9498
JCM 5824
JCM 5827
JCM 5826
JCM 6567
JCM 1432
JCM 10188
JCM 5825 |