RRC ID 41187
著者 Sugahara H, Odamaki T, Fukuda S, Kato T, Xiao JZ, Abe F, Kikuchi J, Ohno H.
タイトル Probiotic Bifidobacterium longum alters gut luminal metabolism through modification of the gut microbial community.
ジャーナル Sci Rep
Abstract Probiotics are well known as health-promoting agents that modulate intestinal microbiota. However, the molecular mechanisms underlying this effect remain unclear. Using gnotobiotic mice harboring 15 strains of predominant human gut-derived microbiota (HGM), we investigated the effects of Bifidobacterium longum BB536 (BB536-HGM) supplementation on the gut luminal metabolism. Nuclear magnetic resonance (NMR)-based metabolomics showed significantly increased fecal levels of pimelate, a precursor of biotin, and butyrate in the BB536-HGM group. In addition, the bioassay revealed significantly elevated fecal levels of biotin in the BB536-HGM group. Metatranscriptomic analysis of fecal microbiota followed by an in vitro bioassay indicated that the elevated biotin level was due to an alteration in metabolism related to biotin synthesis by Bacteroides caccae in this mouse model. Furthermore, the proportion of Eubacterium rectale, a butyrate producer, was significantly higher in the BB536-HGM group than in the group without B. longum BB536 supplementation. Our findings help to elucidate the molecular basis underlying the effect of B. longum BB536 on the gut luminal metabolism through its interactions with the microbial community.
巻・号 5
ページ 13548
公開日 2015-8-28
DOI 10.1038/srep13548
PII srep13548
PMID 26315217
PMC PMC4552000
MeSH Animals Bifidobacterium / drug effects Bifidobacterium / genetics Bifidobacterium / metabolism* Biotin / metabolism Feces / microbiology Gastrointestinal Tract / drug effects Gastrointestinal Tract / microbiology* Gene Expression Regulation / drug effects Germ-Free Life Humans Magnetic Resonance Spectroscopy Metabolome / drug effects Metabolomics Mice Microbiota / drug effects* Probiotics / pharmacology* RNA, Messenger / genetics RNA, Messenger / metabolism Solubility Species Specificity Water
IF 3.998
引用数 63
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 15632 JCM 5828 JCM 9498 JCM 5824 JCM 5827 JCM 5826 JCM 6567 JCM 1432 JCM 10188 JCM 5825