Reference - Detail
|Author||Gribble SL, Nikolaus OB, Dorsky RI.|
|Title||Regulation and function of Dbx genes in the zebrafish spinal cord.|
Dbx homeodomain proteins are important for spinal cord dorsal/ventral patterning and the production of multiple spinal cord cell types. We have examined the regulation and function of Dbx genes in the zebrafish. We report that Hedgehog signaling is not required for the induction or maintenance of these genes; in the absence of Hedgehog signaling, dbx1a/1b/2 are expanded ventrally with concomitant increases in postmitotic neurons that differentiate from this domain. Also, we find that retinoic acid signaling is not required for the induction of Dbx expression. Furthermore, we are the first to report that knockdown of Dbx1 function causes a dorsal expansion of nkx6.2, which is thought to be the cross-repressive partner of Dbx1 in mouse. Our data confirm that the dbx1a/1b/2 domain in zebrafish spinal cord development behaves similarly to amniotes, while extending knowledge of Dbx1 function in spinal cord patterning.
|MeSH||Animals Base Sequence Body Patterning / genetics Embryonic Stem Cells / cytology Embryonic Stem Cells / metabolism Gene Expression Regulation, Developmental Genes, Homeobox* Hedgehog Proteins / genetics Hedgehog Proteins / metabolism Homeodomain Proteins / antagonists & inhibitors Homeodomain Proteins / genetics Neuroglia / cytology Neuroglia / metabolism Neurons / cytology Neurons / metabolism Oligodeoxyribonucleotides, Antisense / genetics Signal Transduction Spinal Cord / cytology Spinal Cord / embryology* Spinal Cord / metabolism* Transcription Factors / antagonists & inhibitors Transcription Factors / genetics Tretinoin / metabolism Zebrafish / embryology* Zebrafish / genetics* Zebrafish / metabolism Zebrafish Proteins / antagonists & inhibitors Zebrafish Proteins / genetics|
|WOS Category||ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY|