論文 - 詳細
| RRC ID | 41261 |
|---|---|
| 著者 | Hirakata Y, Oshiki M, Kuroda K, Hatamoto M, Kubota K, Yamaguchi T, Harada H, Araki N. |
| タイトル | Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge. |
| ジャーナル | Microbes Environ |
| Abstract |
The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA gene sequences from the prokaryotes Methanoregula boonei and Clostridium aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these prokaryotes were localized within Metopus cells. These results identify M. boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus ciliates. |
| 巻・号 | 30(4) |
| ページ | 335-8 |
| 公開日 | 2015-1-1 |
| DOI | 10.1264/jsme2.ME15154 |
| PMID | 26639580 |
| PMC | PMC4676557 |
| MeSH | Biota* Ciliophora / microbiology* Clostridium / classification Clostridium / genetics Clostridium / isolation & purification* Cluster Analysis DNA, Archaeal / chemistry DNA, Archaeal / genetics DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics In Situ Hybridization, Fluorescence Methanomicrobiales / classification Methanomicrobiales / genetics Methanomicrobiales / isolation & purification* Microbial Consortia Microscopy, Electron, Transmission Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sewage / microbiology* Symbiosis* |
| IF | 2.575 |
| 引用数 | 14 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 3805 |