論文 - 詳細
| RRC ID | 41283 |
|---|---|
| 著者 | Koh HW, Song HS, Song U, Yim KJ, Roh SW, Park SJ. |
| タイトル | Halolamina sediminis sp. nov., an extremely halophilic archaeon isolated from solar salt. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
An extremely halophilic archaeal strain, halo-7T, was isolated from brine sediment of the Gomso solar saltern, Republic of Korea. Cells of strain halo-7T were pleomorphic, stained Gram-negative, lysed in distilled water and formed red-pigmented colonies. Strain halo-7T grew in the range of 25-45°C (optimum 37-40°C), pH 6.5-9.5 (optimum pH 7.0-8.0), 15-30% (w/v) NaCl (optimum, 20-25%), and 0.05-0.5 M MgCl2 (optimum 0.1-0.3 M). The minimal NaCl concentration to prevent cell lysis of strain halo-7T was 10% (w/v). The major polar lipids of the isolate were phosphatidylglycerol, phosphatidylglycerolphosphate methyl ester, an unidentified phospholipid, an unidentified lipid, and two unidentified glycolipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain halo-7T is closely related to the members of the genus Halolamina, Halolamina salina WSY15-H3T (98.7% 16S rRNA gene sequence similarity), Halolamina pelagica TBN21T (98.2%) and Halolamina rubra CBA1107T (97.4%). The genomic DNA G+C content determined for strain halo-7T (68.0 mol%) was slightly higher than those of H. salina JCM 18549T and H. rubra CBA1107T. DNA-DNA hybridization values between strain halo-7T and reference strains were < 25%. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe a novel species of the genus Halolamina, represented by strain halo-7T, for which we propose the name Halolamina sediminis sp. nov. The type strain is halo-7T ( = JCM 30187T = CECT 8739T). |
| 巻・号 | 65(8) |
| ページ | 2479-2484 |
| 公開日 | 2015-8-1 |
| DOI | 10.1099/ijs.0.000287 |
| PMID | 25920722 |
| MeSH | Base Composition DNA, Archaeal / genetics Glycolipids / chemistry Halobacteriaceae / classification* Halobacteriaceae / genetics Halobacteriaceae / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Republic of Korea Salts* Sequence Analysis, DNA Sodium Chloride* |
| IF | 2.166 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 16809 JCM 18548 JCM 18549 JCM 30187 |