Reference - Detail
| RRC ID | 41308 |
|---|---|
| Author | Higashi Y, Sokawa Y, Watanabe Y, Kawade Y, Ohno S, Takaoka C, Taniguchi T. |
| Title | Structure and expression of a cloned cDNA for mouse interferon-beta. |
| Journal | J Biol Chem |
| Abstract |
A unique sequence in the mouse genome which cross-hybridized to a cloned human interferon-beta 1 gene was detected by DNA blot analysis. Taking advantage of this, a cDNA library prepared from partially purified mRNA for mouse interferon-beta was screened using human interferon-beta 1 DNA as a probe. One of the positive clones, pM beta-3, contained a 680-base pair cDNA insert, whose base sequence contained a single large open reading frame for 182 amino acids. The coding sequences of the cDNA showed homologies of 63% at the nucleotide and 48% at the amino acid level with respect to human interferon-beta 1 cDNA (Taniguchi, T., Ohno, S., Fujii-Kuriyama, Y., and Muramatsu, M. (1980) Gene 10, 11-15). The first 21 amino acids, considered to be the signal peptide, were followed by 24 amino acids, whose sequence was identical with the NH2-terminal sequence that had been reported for mouse interferon-beta from Ehrlich ascites tumor cells (Taira, H., Broeze, R. J., Jayaram, B. M., Lengyel, P., Hunkapiller, M. W., and Hood, L. E. (1980) Science (Wash. D.C.) 207, 528-530). The complete primary sequence of mature interferon-beta polypeptide consisting of 161 amino acids (Mr = 19,700) was deduced. There are three N-glycosylation sites, and this offers an explanation for the larger molecular size (Mr = 26,000-40,000) of natural mouse interferon-beta in comparison to the deduced interferon polypeptide. The cDNA, when fused to a SV40 promoter sequence and then introduced into COS-7 cells, directed the synthesis and secretion of a protein product indistinguishable from the authentic mouse interferon-beta. |
| Volume | 258(15) |
| Pages | 9522-9 |
| Published | 1983-8-10 |
| PII | S0021-9258(17)44698-9 |
| PMID | 6688252 |
| MeSH | Amino Acid Sequence Base Sequence Cloning, Molecular DNA / analysis* Gene Expression Regulation* Interferon Type I / genetics* Nucleic Acid Hybridization |
| IF | 4.238 |
| Times Cited | 159 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 26 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pCMV (Hu beta) (RDB01681) |