Reference - Detail
| RRC ID | 41315 |
|---|---|
| Author | Sakai H, Sumitani M, Chikami Y, Yahata K, Uchino K, Kiuchi T, Katsuma S, Aoki F, Sezutsu H, Suzuki MG. |
| Title | Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori. |
| Journal | PLoS Genet |
| Abstract |
In Bombyx mori (B. mori), Fem piRNA originates from the W chromosome and is responsible for femaleness. The Fem piRNA-PIWI complex targets and cleaves mRNAs transcribed from the Masc gene. Masc encodes a novel CCCH type zinc-finger protein and is required for male-specific splicing of B. mori doublesex (Bmdsx) transcripts. In the present study, several silkworm strains carrying a transgene, which encodes a Fem piRNA-resistant Masc mRNA (Masc-R), were generated. Forced expression of the Masc-R transgene caused female-specific lethality during the larval stages. One of the Masc-R strains weakly expressed Masc-R in various tissues. Females heterozygous for the transgene expressed male-specific isoform of the Bombyx homolog of insulin-like growth factor II mRNA-binding protein (ImpM) and Bmdsx. All examined females showed a lower inducibility of vitellogenin synthesis and exhibited abnormalities in the ovaries. Testis-like tissues were observed in abnormal ovaries and, notably, the tissues contained considerable numbers of sperm bundles. Homozygous expression of the transgene resulted in formation of the male-specific abdominal segment in adult females and caused partial male differentiation in female genitalia. These results strongly suggest that Masc is an important regulatory gene of maleness in B. mori. |
| Volume | 12(8) |
| Pages | e1006203 |
| Published | 2016-8-1 |
| DOI | 10.1371/journal.pgen.1006203 |
| PII | PGENETICS-D-16-00853 |
| PMID | 27579676 |
| PMC | PMC5007099 |
| MeSH | Animals Animals, Genetically Modified Bombyx / genetics* Bombyx / growth & development DNA-Binding Proteins / biosynthesis DNA-Binding Proteins / genetics* Female Gene Expression Regulation, Developmental Insect Proteins / biosynthesis Insect Proteins / genetics* Insulin-Like Growth Factor II / biosynthesis Insulin-Like Growth Factor II / genetics* Male Ovary / growth & development Ovary / metabolism RNA Splicing / genetics RNA, Small Interfering / biosynthesis RNA, Small Interfering / genetics* Sex Chromosomes / genetics Sex Determination Processes / genetics* Testis / growth & development Testis / metabolism Vitellogenins / biosynthesis Vitellogenins / genetics |
| IF | 5.175 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | Suzu19-1 |