Reference - Detail
| RRC ID | 41326 |
|---|---|
| Author | Soeda S, Honda O, Shimeno H. |
| Title | Vascular endothelial cell monolayer formed on membrane filter potentiates the defense against tumor cell invasion by treatment with brefeldin A. |
| Journal | Cancer Lett |
| Abstract |
We constructed vascular endothelial cell monolayer on a fibronectin-coated filter in a Boyden chamber and assessed the ability of 3 LL cells to penetrate through the artificial blood vessel wall. The defense of endothelial cell monolayers against the tumor cell invasion was greatly potentiated by their pretreatment with 5 or 10 micrograms/ml of brefeldin A (BFA) for 1 h (52% or 28% of control invasion). Treatment of the endothelial cell monolayers with BFA resulted in an increase in the release of inhibitory material(s) against urokinase-type plasminogen activator (u-PA) activity of 3 LL cells. Parallel experiments with the cultured endothelial cells and BFA indicated that the fungal metabolite enhanced a rate of accumulation of plasminogen activator inhibitor-1 (PAI-1) antigen, but not of tissue-type plasminogen activator antigen in the medium. The BFA-induced enhancement of PAI-1 antigen release was accompanied with the increased accumulation of the extracellular (membrane/matrix-bound) and intracellular PAI-1 antigen (219% of control at 24 h). These results suggest that BFA can strengthen the defense of vascular endothelium against tumor-cell invasion by enhancing the release and accumulation of PAI-1, which plays a critical role in the regulation of the u-PA-plasmin-collagenase activation cascade. |
| Volume | 108(1) |
| Pages | 49-54 |
| Published | 1996-11-12 |
| DOI | 10.1016/s0304-3835(96)04392-3 |
| PII | S0304383596043923 |
| PMID | 8950208 |
| MeSH | Brefeldin A Cell Adhesion / drug effects Cells, Cultured Collagenases / metabolism Cyclopentanes / pharmacology* Endothelium, Vascular / cytology Endothelium, Vascular / drug effects* Endothelium, Vascular / metabolism Extracellular Matrix / metabolism Humans Neoplasm Invasiveness / prevention & control* Plasminogen Activator Inhibitor 1 / metabolism* Tissue Plasminogen Activator / metabolism Tumor Cells, Cultured Umbilical Veins Urokinase-Type Plasminogen Activator / metabolism* |
| Resource | |
| Human and Animal Cells | LLC(RCB0588) |